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Co-expression of cancer driver genes: IDH-wildtype glioblastoma-derived tumorspheres

DC FieldValueLanguage
dc.contributor.author강석구-
dc.contributor.author김세훈-
dc.contributor.author김의현-
dc.contributor.author문주형-
dc.contributor.author박상욱-
dc.contributor.author손혜영-
dc.contributor.author윤선진-
dc.contributor.author장종희-
dc.contributor.author허용민-
dc.date.accessioned2021-01-19T07:56:43Z-
dc.date.available2021-01-19T07:56:43Z-
dc.date.issued2020-12-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/181387-
dc.description.abstractBackground: Driver genes of GBM may be crucial for the onset of isocitrate dehydrogenase (IDH)-wildtype (WT) glioblastoma (GBM). However, it is still unknown whether the genes are expressed in the identical cluster of cells. Here, we have examined the gene expression patterns of GBM tissues and patient-derived tumorspheres (TSs) and aimed to find a progression-related gene. Methods: We retrospectively collected primary IDH-WT GBM tissue samples (n = 58) and tumor-free cortical tissue samples (control, n = 20). TSs are isolated from the IDH-WT GBM tissue with B27 neurobasal medium. Associations among the driver genes were explored in the bulk tissue, bulk cell, and a single cell RNAsequencing techniques (scRNAseq) considering the alteration status of TP53, PTEN, EGFR, and TERT promoter as well as MGMT promoter methylation. Transcriptomic perturbation by temozolomide (TMZ) was examined in the two TSs. Results: We comprehensively compared the gene expression of the known driver genes as well as MGMT, PTPRZ1, or IDH1. Bulk RNAseq databases of the primary GBM tissue revealed a significant association between TERT and TP53 (p < 0.001, R = 0.28) and its association increased in the recurrent tumor (p < 0.001, R = 0.86). TSs reflected the tissue-level patterns of association between the two genes (p < 0.01, R = 0.59, n = 20). A scRNAseq data of a TS revealed the TERT and TP53 expressing cells are in a same single cell cluster. The driver-enriched cluster dominantly expressed the glioma-associated long noncoding RNAs. Most of the driver-associated genes were downregulated after TMZ except IGFBP5. Conclusions: GBM tissue level expression patterns of EGFR, TERT, PTEN, IDH1, PTPRZ1, and MGMT are observed in the GBM TSs. The driver gene-associated cluster of the GBM single cells were enriched with the glioma-associated long noncoding RNAs.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherBioMed Central-
dc.relation.isPartOfJOURNAL OF TRANSLATIONAL MEDICINE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleCo-expression of cancer driver genes: IDH-wildtype glioblastoma-derived tumorspheres-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Neurosurgery (신경외과학교실)-
dc.contributor.googleauthorSeon-Jin Yoon-
dc.contributor.googleauthorHye Young Son-
dc.contributor.googleauthorJin-Kyoung Shim-
dc.contributor.googleauthorJu Hyung Moon-
dc.contributor.googleauthorEui-Hyun Kim-
dc.contributor.googleauthorJong Hee Chang-
dc.contributor.googleauthorWan Yee Teo-
dc.contributor.googleauthorSe Hoon Kim-
dc.contributor.googleauthorSahng Wook Park-
dc.contributor.googleauthorYong-Min Huh-
dc.contributor.googleauthorSeok-Gu Kang-
dc.identifier.doi10.1186/s12967-020-02647-8-
dc.contributor.localIdA00036-
dc.contributor.localIdA00610-
dc.contributor.localIdA00610-
dc.contributor.localIdA00837-
dc.contributor.localIdA00837-
dc.contributor.localIdA01383-
dc.contributor.localIdA01383-
dc.contributor.localIdA01487-
dc.contributor.localIdA01487-
dc.contributor.localIdA04589-
dc.contributor.localIdA04589-
dc.contributor.localIdA05256-
dc.contributor.localIdA05256-
dc.contributor.localIdA03470-
dc.contributor.localIdA03470-
dc.contributor.localIdA04359-
dc.contributor.localIdA04359-
dc.relation.journalcodeJ01915-
dc.identifier.eissn1479-5876-
dc.identifier.pmid33317554-
dc.subject.keywordIsocitrate dehydrogenase-wildtype glioblastoma-
dc.subject.keywordSingle cell RNAseq-
dc.subject.keywordTranscriptome-
dc.subject.keywordTumorsphere-
dc.contributor.alternativeNameKang, Seok Gu-
dc.contributor.affiliatedAuthor강석구-
dc.contributor.affiliatedAuthor김세훈-
dc.contributor.affiliatedAuthor김세훈-
dc.contributor.affiliatedAuthor김의현-
dc.contributor.affiliatedAuthor김의현-
dc.contributor.affiliatedAuthor문주형-
dc.contributor.affiliatedAuthor문주형-
dc.contributor.affiliatedAuthor박상욱-
dc.contributor.affiliatedAuthor박상욱-
dc.contributor.affiliatedAuthor손혜영-
dc.contributor.affiliatedAuthor손혜영-
dc.contributor.affiliatedAuthor윤선진-
dc.contributor.affiliatedAuthor윤선진-
dc.contributor.affiliatedAuthor장종희-
dc.contributor.affiliatedAuthor장종희-
dc.contributor.affiliatedAuthor허용민-
dc.contributor.affiliatedAuthor허용민-
dc.citation.volume18-
dc.citation.number1-
dc.citation.startPage482-
dc.identifier.bibliographicCitationJOURNAL OF TRANSLATIONAL MEDICINE, Vol.18(1) : 482, 2020-12-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers

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