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High-speed combined reflectance confocal and moxifloxacin based two-photon microscopy

Authors
 Bumju Kim  ;  Hoan Le  ;  Byung-Ho Oh  ;  Ki Hean Kim 
Citation
 BIOMEDICAL OPTICS EXPRESS, Vol.11(3) : 1555-1567, 2020-02 
Journal Title
 BIOMEDICAL OPTICS EXPRESS 
Issue Date
2020-02
Abstract
Reflectance confocal microscopy (RCM) is a non-invasive high-resolution optical imaging technique used in clinical settings as a diagnostic method. However, RCM has limited diagnostic ability by providing non-specific morphological information only based on reflection contrast. Various multimodal imaging techniques have been developed to compensate the limitations of RCM, but multimodal techniques are often slow in imaging speed compared to RCM alone. In this report, we combined RCM with moxifloxacin based two-photon microscopy (TPM) for high-speed multimodal imaging. Moxifloxacin based TPM used clinically compatible moxifloxacin for cell labeling and could do non-invasive cellular imaging at 30 frames/s together with RCM. Performance of the combined microscopy was characterized in the imaging of mouse skin and cornea, in vivo. Detail tissue microstructures including cells, extra-cellular matrix (ECM), and vasculature were visualized. The combined microscopy was applied to human skin cancer specimens, and both cells and ECM in the skin cancer and normal skin regions were visualized at high imaging speeds. The combined microscopy can be useful in the clinical applications of RCM by providing multiple contrasts.
Files in This Item:
T202005364.pdf Download
DOI
10.1364/BOE.385763
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Oh, Byung Ho(오병호) ORCID logo https://orcid.org/0000-0001-9575-5665
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/181297
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