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Carbon monoxide-releasing molecule-3: Amelioration of renal ischemia reperfusion injury in a rat model

Authors
 Dae Keun Kim 1 , Su-Jin Shin 2 , Jiyoung Lee 3 , Sung Yul Park 3 , Yong Tae Kim 3 , Hong Yong Choi 3 , Young Eun Yoon 3 , Hong Sang Moon 3 
Citation
 INVESTIGATIVE AND CLINICAL UROLOGY, Vol.61(4) : 441-451, 2020-07 
Journal Title
 INVESTIGATIVE AND CLINICAL UROLOGY 
ISSN
 2466-0493 
Issue Date
2020-07
Keywords
Carbon monoxide ; Ischemia ; Kidney diseases
Abstract
Purpose: Despite the role of carbon monoxide in ameliorating ischemia-reperfusion injury (IRI), its use in the clinical setting is restricted owing to its toxicity. Herein, we investigated the in vivo effects of carbon monoxide-releasing molecule-3 (CORM-3) on IRI. Materials and methods: Fifteen rats were equally and randomly divided into three groups: sham (right nephrectomy), control (right nephrectomy and left renal ischemia), and CORM-3 (right nephrectomy and CORM-3 injection before left renal ischemia). Kidney tissues and blood samples collected from sacrificed rats were evaluated to determine the renoprotective effect and mechanism of CORM-3. Results: Concentrations of serum creatinine and kidney injury molecule-1 in the CORM-3 group were significantly lower than in the control group after 75 minutes of IRI (1.2 vs. 2.4 mg/dL, p=0.01, and 292 vs. 550 pg/mL, p<0.001, respectively). Furthermore, the CORM-3 group exhibited a higher portion of normal tubules and glomeruli. TUNEL staining revealed fewer apoptotic renal tubular cells in the CORM-3 group than in the control group. The expression of 960 genes in the CORM-3 group was also altered. Pretreatment with CORM-3 before renal IRI produced a significant renoprotective effect. Fifteen of the altered genes were found to be involved in the peroxisome proliferator-activated receptors signaling pathway, and the difference in the expression of these genes between the CORM-3 and control groups was statistically significant (p<0.001). Conclusions: CORM-3 ameliorates IRI by decreasing apoptosis and may be a novel strategy for protection against renal warm IRI.
Files in This Item:
T202005040.pdf Download
DOI
10.4111/icu.2020.61.4.441
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Shin, Su Jin(신수진) ORCID logo https://orcid.org/0000-0001-9114-8438
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/180699
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