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Aggregation-driven fluorescence quenching of imidazole-functionalized perylene diimide for urea sensing

Authors
 Junghyun Cho  ;  Changjoon Keum  ;  Sang-Guk Lee  ;  Sang-Yup Lee 
Citation
 ANALYST, Vol.145(22) : 7312-7319, 2020-11 
Journal Title
 ANALYST 
ISSN
 0003-2654 
Issue Date
2020-11
Abstract
Stimuli-responsive self-assembly of functional amphiphilic molecules by specific chemical stimulants is a promising strategy for sensor application. Herein, we demonstrate a fast optical detection of urea in human urine by exploiting bolaform perylene diimide functionalized with imidazoles (PDI-Hm), whose aggregation is induced by urea hydrolysis. The hydroxides produced from the enzymatic urea hydrolysis deprotonate the imidazoles to reduce electrostatic repulsion between PDI-Hm molecules in a HCl-methanol mixture, thereby leading to aggregation and consequent fluorescence quenching. The molecular interaction of PDI-Hm was further scrutinized to understand the aggregation behavior driven by the screening of electrical repulsion. As an optical sensing probe, PDI-Hm displays a prompt response (<1 min) to hydroxide and detection limit of 0.4 mM for urea. PDI-Hm incorporating urease offers considerable selectivity toward urea among various components in human urine. The urea sensing accuracy of this PDI-Hm fluorescence chemosensor is comparable to that of a clinical method, showing 93.4% consistency. Furthermore, the PDI-Hm was fabricated into a gel film allowed for the fast screening of excessive urea in urine.
Full Text
https://pubs.rsc.org/en/content/articlelanding/2020/AN/D0AN01252A#!divAbstract
DOI
10.1039/d0an01252a
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Sang-Guk(이상국) ORCID logo https://orcid.org/0000-0003-3862-3660
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/180612
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