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Application of single-cell RNA sequencing on human skin: Technical evolution and challenges

Authors
 Doyoung Kim  ;  Kyung Bae Chung  ;  Tae-Gyun Kim 
Citation
 JOURNAL OF DERMATOLOGICAL SCIENCE, Vol.99(2) : 74-81, 2020-08 
Journal Title
 JOURNAL OF DERMATOLOGICAL SCIENCE 
ISSN
 0923-1811 
Issue Date
2020-08
Keywords
Cellular heterogeneity ; Next-generation sequencing ; Single-cell RNA sequencing ; Transcriptome
Abstract
The bulk tissue RNA sequencing technique measures the average gene expression of potentially heterogeneous cellular subsets of human skin. However, single-cell RNA sequencing (scRNA-seq) enables both profiling of gene expression measurements at a single-cell resolution and identification of cellular heterogeneity. This recent technical advance has broadened the understanding of many aspects of skin biology, such as development, oncogenesis, and immunopathogenesis. However, due to the low number of mRNAs detectable in an individual cell and the alteration of transcriptomes during sample preparation, scRNA-seq data are often extremely noisy. Moreover, unstandardized methodologies for sample preparation, capturing, and bioinformatic analysis (e.g., batch correction or integration) hamper reliable inter-study comparisons. Nevertheless, sophisticated bioinformatic analysis and integrative omics-based approaches are making up for these limitations. Here, we discuss both the advantages and technical challenges of scRNA-seq, a promising tool opening new horizons in dermatological research.
Full Text
https://www.sciencedirect.com/science/article/pii/S0923181120301985
DOI
10.1016/j.jdermsci.2020.06.002
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Do Young(김도영) ORCID logo https://orcid.org/0000-0002-0194-9854
Kim, Tae-Gyun(김태균) ORCID logo https://orcid.org/0000-0002-2116-4579
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/179968
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