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Cell-mimic polymersome-shielded islets for long-term immune protection of neonatal porcine islet-like cell clusters

Authors
 Hyun-Ouk Kim  ;  Sang Hoon Lee  ;  Woonsung Na  ;  Jong-Woo Lim  ;  Geunseon Park  ;  Chaewon Park  ;  Hwunjae Lee  ;  Aram Kang  ;  Seungjoo Haam  ;  Inho Choi  ;  Jung-Taek Kang  ;  Daesub Song 
Citation
 JOURNAL OF MATERIALS CHEMISTRY B, Vol.8(12) : 2476-2482, 2020-03 
Journal Title
 JOURNAL OF MATERIALS CHEMISTRY B 
ISSN
 2050-750X 
Issue Date
2020-03
Abstract
Although islet cell transplantation has emerged as a promising treatment for type 1 diabetes, it remains an unmet clinical application due to the need for immunosuppression to prevent islet elimination and autoimmunity. To solve these problems, we developed novel nanoencapsulation of neonatal porcine islet-like cell clusters (NPCCs) with cell-mimic polymersomes (PSomes) based on PEG-b-PLA (poly(ethylene glycol)-b-poly(dl-lactic acid)). To accomplish this, we first formulated NHS-, NH2-, COOH-, and m(methoxy)-PSomes. This coating utilizes interactions involving NPCC surfaces and PSomes that have covalent bonds, electrostatic interactions, and hydrogen bonds. We extended the range of applicability by comparing the binding affinity of electrostatic attraction and hydrogen bonding, as well as covalent bonds. Our protocol can be used as an efficient hydrogen bonding method because it reduces cell membrane damage as well as the use of covalent bonding methods. We verified the selective permeability of NHS-, NH2-, COOH-, and m-PSome-shielded NPCCs. Furthermore, we showed that a novel nanoencapsulation did not affect insulin secretion from NPCCs. This study offers engineering advances in islet encapsulation technologies to be used for cell-based transplantation therapies.
Full Text
https://pubs.rsc.org/en/content/articlelanding/2020/TB/C9TB02270H#!divAbstract
DOI
10.1039/c9tb02270h
Appears in Collections:
5. Research Institutes (연구소) > Institute for Medical Convergence (연의-생공연 메디컬융합연구소) > 1. Journal Papers
Yonsei Authors
Lee, Hwun Jae(이훈재)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/179923
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