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New staining method using methionyl-tRNA synthetase 1 antibody for brushing cytology of bile duct cancer

Authors
 Sung Ill Jang  ;  Nam Hoon Kwon  ;  Beom Jin Lim  ;  Ji Hae Nahm  ;  Joon Seong Park  ;  Chang Moo Kang  ;  So Ra Park  ;  Su Yun Lee Sd  ;  Beom Sik Kang  ;  Sunghoon Kim  ;  Dong Ki Lee 
Citation
 GASTROINTESTINAL ENDOSCOPY, Vol.92(2) : 310-319, 2020-08 
Journal Title
 GASTROINTESTINAL ENDOSCOPY 
ISSN
 0016-5107 
Issue Date
2020-08
Abstract
Background and aims: Identifying malignant biliary strictures using endobiliary brushing cytology specimens is important for treatment decision-making and prognosis prediction. The sensitivity of brushing cytology specimens based on Papanicolaou (Pap) staining is low, which hampers accurate diagnosis of indeterminate strictures. Here, we assessed the diagnostic value of immunohistochemical (IHC) and immunofluorescence (IF) staining for methionyl-tRNA synthetase 1 (MARS1). Methods: Endobiliary brushing cytology specimens were obtained during ERCP from 80 patients with an extrahepatic biliary stricture. Pap and MARS1 IF staining were performed on liquid-based cytology slides derived from these specimens. Sections of bile duct adenocarcinoma and normal bile duct tissue were obtained from 45 patients who underwent surgery for malignant biliary stricture, and MARS1 levels were evaluated by IHC staining. Results: MARS1 IF staining was applied to brushing cytology specimens, and the results showed strong signals in malignant biliary structures but not in the negative for malignancy specimens. Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 70.4%, 96.2%, 97.4%, 56.8%, and 78.8%, respectively, for conventional Pap staining and 98.1%, 96.1%, 98.1%, 96.2%, and 97.5%, respectively, for MARS1 IF (P < .0001). When IHC staining was used, MARS1 was detected in 45 bile duct adenocarcinoma sections but not in 15 normal bile duct sections. Moreover, MARS1 mRNA and protein levels were significantly higher in bile duct adenocarcinoma sections according to polymerase chain reaction and Western blot, respectively. Conclusions: The high sensitivity and accuracy of MARS1 IF staining enabled detection of malignancy in patients with indeterminate biliary stricture. Further prospective studies are needed to validate our findings. (Clinical trial registration number: KCT 0003285.).
Full Text
https://www.sciencedirect.com/science/article/pii/S0016510719325593
DOI
10.1016/j.gie.2019.12.017
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Chang Moo(강창무) ORCID logo https://orcid.org/0000-0002-5382-4658
Nahm, Ji Hae(남지해) ORCID logo https://orcid.org/0000-0003-0902-866X
Park, Joon Seong(박준성) ORCID logo https://orcid.org/0000-0001-8048-9990
Lee, Dong Ki(이동기) ORCID logo https://orcid.org/0000-0002-0048-9112
Lim, Beom Jin(임범진) ORCID logo https://orcid.org/0000-0003-2856-0133
Jang, Sung Ill(장성일) ORCID logo https://orcid.org/0000-0003-4937-6167
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/179697
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