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New staining method using methionyl-tRNA synthetase 1 antibody for brushing cytology of bile duct cancer

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dc.contributor.author이동기-
dc.contributor.author장성일-
dc.contributor.author남지해-
dc.contributor.author임범진-
dc.contributor.author박준성-
dc.contributor.author강창무-
dc.date.accessioned2020-10-04T16:51:42Z-
dc.date.available2020-10-04T16:51:42Z-
dc.date.issued2020-08-
dc.identifier.issn0016-5107-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/179697-
dc.description.abstractBackground and aims: Identifying malignant biliary strictures using endobiliary brushing cytology specimens is important for treatment decision-making and prognosis prediction. The sensitivity of brushing cytology specimens based on Papanicolaou (Pap) staining is low, which hampers accurate diagnosis of indeterminate strictures. Here, we assessed the diagnostic value of immunohistochemical (IHC) and immunofluorescence (IF) staining for methionyl-tRNA synthetase 1 (MARS1). Methods: Endobiliary brushing cytology specimens were obtained during ERCP from 80 patients with an extrahepatic biliary stricture. Pap and MARS1 IF staining were performed on liquid-based cytology slides derived from these specimens. Sections of bile duct adenocarcinoma and normal bile duct tissue were obtained from 45 patients who underwent surgery for malignant biliary stricture, and MARS1 levels were evaluated by IHC staining. Results: MARS1 IF staining was applied to brushing cytology specimens, and the results showed strong signals in malignant biliary structures but not in the negative for malignancy specimens. Sensitivity, specificity, positive predictive value, negative predictive value, and accuracy were 70.4%, 96.2%, 97.4%, 56.8%, and 78.8%, respectively, for conventional Pap staining and 98.1%, 96.1%, 98.1%, 96.2%, and 97.5%, respectively, for MARS1 IF (P < .0001). When IHC staining was used, MARS1 was detected in 45 bile duct adenocarcinoma sections but not in 15 normal bile duct sections. Moreover, MARS1 mRNA and protein levels were significantly higher in bile duct adenocarcinoma sections according to polymerase chain reaction and Western blot, respectively. Conclusions: The high sensitivity and accuracy of MARS1 IF staining enabled detection of malignancy in patients with indeterminate biliary stricture. Further prospective studies are needed to validate our findings. (Clinical trial registration number: KCT 0003285.).-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherMosby Yearbook-
dc.relation.isPartOfGASTROINTESTINAL ENDOSCOPY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleNew staining method using methionyl-tRNA synthetase 1 antibody for brushing cytology of bile duct cancer-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthorSung Ill Jang-
dc.contributor.googleauthorNam Hoon Kwon-
dc.contributor.googleauthorBeom Jin Lim-
dc.contributor.googleauthorJi Hae Nahm-
dc.contributor.googleauthorJoon Seong Park-
dc.contributor.googleauthorChang Moo Kang-
dc.contributor.googleauthorSo Ra Park-
dc.contributor.googleauthorSu Yun Lee Sd-
dc.contributor.googleauthorBeom Sik Kang-
dc.contributor.googleauthorSunghoon Kim-
dc.contributor.googleauthorDong Ki Lee-
dc.identifier.doi10.1016/j.gie.2019.12.017-
dc.contributor.localIdA02723-
dc.contributor.localIdA03441-
dc.contributor.localIdA05120-
dc.contributor.localIdA03363-
dc.contributor.localIdA01672-
dc.contributor.localIdA00088-
dc.relation.journalcodeJ00920-
dc.identifier.eissn1097-6779-
dc.identifier.pmid31874158-
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S0016510719325593-
dc.contributor.alternativeNameLee, Dong Ki-
dc.contributor.affiliatedAuthor이동기-
dc.contributor.affiliatedAuthor장성일-
dc.contributor.affiliatedAuthor남지해-
dc.contributor.affiliatedAuthor임범진-
dc.contributor.affiliatedAuthor박준성-
dc.contributor.affiliatedAuthor강창무-
dc.citation.volume92-
dc.citation.number2-
dc.citation.startPage310-
dc.citation.endPage319-
dc.identifier.bibliographicCitationGASTROINTESTINAL ENDOSCOPY, Vol.92(2) : 310-319, 2020-08-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers

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