Efficacy and Gut Dysbiosis of Gentamicin-Intercalated Smectite as a New Therapeutic Agent againstHelicobacter pyloriin a Mouse Model

Abstract

Helicobacter pylorieradication rate with conventional standard therapy is decreasing owing to antibiotic resistance, necessitating novel antibacterial strategies againstH. pylori. We evaluated the efficacy of a gentamicin-intercalated smectite hybrid (S-GM)-based treatment and analyzed fecal microbiome composition inH. pylori-infected mice. To evaluate anti-H. pyloriefficacy, mice were divided into eight groups, andH. pylorieradication was assessed by aCampylobacter-like organism (CLO) test and PCR assay ofH. pyloriin gastric mucosa. One week afterH. pylorieradication, pro-inflammatory cytokine levels and atrophic changes in gastric mucosa were examined. Stool specimens were collected and analyzed for microbiome changes. The S-GM-based triple regimen decreased bacterial burden in vivo, compared with that in untreated mice or mice treated with other regimens. The therapeutic reactions in the CLO test from gastric mucosa were both 90% in the standard triple therapy and S-GM therapy group, respectively. Those ofH. pyloriPCR in mouse gastric mucosa were significantly lower in standard triple therapy and S-GM therapy groups than in the non-treatment group. Toxicity test results showed that S-GM therapy reduced IL-8 level and atrophic changes in gastric mucosa. Stool microbiome analysis revealed that compared with mice treated with the standard triple therapy, mice treated with the S-GM therapy showed microbiome diversity and abundant microorganisms at the phylum level. Our results suggested that S-GM is a promising and effective therapeutic agent againstH. pyloriinfection.