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Clinicopathological parameters for circulating tumor DNA shedding in surgically resected non-small cell lung cancer with EGFR or KRAS mutation

DC Field Value Language
dc.contributor.author박철환-
dc.contributor.author이성수-
dc.date.accessioned2020-09-28T02:02:37Z-
dc.date.available2020-09-28T02:02:37Z-
dc.date.issued2020-03-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/179037-
dc.description.abstractBackground: Circulating tumor DNA (ctDNA) is cell-free DNA that is released into peripheral blood by tumor cells. ctDNA harbors somatic mutations and mutant ctDNA obtained from blood can be used as a biomarker in advanced non-small cell lung cancer (NSCLC). In this study, we investigated the clinicopathological properties of tumors that shed ctDNA in surgically resected NSCLC patients. Methods: Consecutive cases of NSCLC with matching surgically resected tissue specimens and peripheral or specimen blood samples were eligible for this study. EGFR and KRAS mutations in plasma ctDNA and formalin-fixed paraffin-embedded tissue were analyzed using peptide nucleic acid clamping-assisted method. The plasma and tissue results were compared according to clinicopathological features. Results: Mutation analyses were available for 36 cases. EGFR and KRAS mutations were present in 41.7% (15/36) and 16.7% (6/36) of tissue samples, respectively. Among EGFR and KRAS-mutant tumors, plasma mutation detection sensitivity was 13.3% (2/15) for EGFR and 33.3% (2/6) for KRAS. The presence of ctDNA in plasma was significantly associated with higher pathological tumor stage (p = 0.028), nodal metastasis (p = 0.016), solid adenocarcinoma pattern (p = 0.003), tumor necrosis (p = 0.012), larger primary tumor diameter (p = 0.002) or volume (p = 0.002), and frequent mitosis (p = 0.018) in tissue specimens. All tumors larger than 4 cm in maximal diameter or 25 cm3 in volume shed ctDNA in plasma. In subgroup analysis among EGFR mutated adenocarcinoma, ctDNA was significantly associated with nodal metastasis (p = 0.029), vascular invasion (p = 0.029), solid adenocarcinoma pattern (p = 0.010), and tumor necrosis (p = 0.010), high mitotic rate (p = 0.009), large pathological tumor size (p = 0.027), and large tumor volume on CT (p = 0.027). Conclusion: We suggest that primary or total tumor burden, solid adenocarcinoma morphology, tumor necrosis, and frequent mitosis could predict ctDNA shedding in pulmonary adenocarcinoma.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherPublic Library of Science-
dc.relation.isPartOfPLOS ONE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAdult-
dc.subject.MESHAged-
dc.subject.MESHAged, 80 and over-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung / metabolism-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung / pathology*-
dc.subject.MESHCarcinoma, Non-Small-Cell Lung / surgery-
dc.subject.MESHCirculating Tumor DNA / blood-
dc.subject.MESHCirculating Tumor DNA / metabolism*-
dc.subject.MESHErbB Receptors / blood-
dc.subject.MESHErbB Receptors / genetics-
dc.subject.MESHFemale-
dc.subject.MESHHumans-
dc.subject.MESHLung Neoplasms / metabolism-
dc.subject.MESHLung Neoplasms / pathology*-
dc.subject.MESHLung Neoplasms / surgery-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHNeoplasm Staging-
dc.subject.MESHPolymorphism, Single Nucleotide-
dc.subject.MESHProto-Oncogene Proteins p21(ras) / blood-
dc.subject.MESHProto-Oncogene Proteins p21(ras) / genetics*-
dc.titleClinicopathological parameters for circulating tumor DNA shedding in surgically resected non-small cell lung cancer with EGFR or KRAS mutation-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Radiology (영상의학교실)-
dc.contributor.googleauthorMin-Sun Cho-
dc.contributor.googleauthorChul Hwan Park-
dc.contributor.googleauthorSungsoo Lee-
dc.contributor.googleauthorHeae Surng Park-
dc.identifier.doi10.1371/journal.pone.0230622-
dc.contributor.localIdA01722-
dc.contributor.localIdA02866-
dc.relation.journalcodeJ02540-
dc.identifier.eissn1932-6203-
dc.identifier.pmid32196518-
dc.contributor.alternativeNamePark, Chul Hwan-
dc.contributor.affiliatedAuthor박철환-
dc.contributor.affiliatedAuthor이성수-
dc.citation.volume15-
dc.citation.number3-
dc.citation.startPagee0230622-
dc.identifier.bibliographicCitationPLOS ONE, Vol.15(3) : e0230622, 2020-03-
dc.identifier.rimsid67549-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers

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