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Production of Inflammatory Cytokines and Nitric Oxide by Human Mast Cells Incubated with Toxoplasma gondii Lysate

Authors
 Eun-Ah Park  ;  Ik-Hwan Han  ;  Jung-Hyun Kim  ;  Soon-Jung Park  ;  Jae-Sook Ryu  ;  Myoung-Hee Ahn 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.57(2) : 201-206, 2019-04 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
2019-04
MeSH
Blotting, Western ; Cells, Cultured ; Complex Mixtures / immunology* ; Cytokines / metabolism* ; Enzyme-Linked Immunosorbent Assay ; Humans ; Mast Cells / metabolism* ; Nitric Oxide / metabolism* ; Real-Time Polymerase Chain Reaction ; Toxoplasma / immunology*
Keywords
Toxoplasma gondii ; cytokine ; mast cell ; nitric oxide
Abstract
The roles of mast cells in allergic diseases and helminth infections are well known. However, the roles of mast cells in T. gondii infection is poorly understood. This study was focused on the production of pro-inflammatory cytokines (TNF-α, IL-4), chemokines (CXCL8, MCP-1) and nitric oxide (NO) by mast cells in response to soluble lysate of T. gondii tachyzoites. Production of CXCL8 (IL-8), MCP-1, TNF-α and IL-4 were measured by RT-PCR and ELISA. Western blot were used for detection of CXCR-1 and CXCR2. Our results showed that T. gondii lysates triggered mast cells to release CXCL8, MCP-1, TNF-α, IL-4 and to produce NO. This suggests that mast cells play an important role in inflammatory responses to T. gondii.
Files in This Item:
T9992019041.pdf Download
DOI
10.3347/kjp.2019.57.2.201
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178947
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