find the monthly variation of kalopanaxsaponin contents in the leaves of Kalopanax pictus with thorns (KPT)kalopanaxsaponin fractions were prepared. KPT collected on May showed the lowest saponin content of the KPTs whereasKPT on August exhibited the highest saponin content. From September, the saponin content in the leaves decreased. Thehighest saponin content was shown to be 7.3% in KPN collected on August. Evaluation of six kalopanaxsaponins A, I, J,B, H and K (KPA, KPI, KPJ, KPB, KPH, and KPK) were performed using TLC densitometer. In this measurement, con-siderably higher KPB and KPH, both hederagenin bisdesmosides, were found whereas very low contents in monodes-mosides KPI and KPJ were observed. In conclusion, it was shown that the leaves of KPN of August could be a biomaterialand the content of KPB as the representative compound for kalopanaxsaponins will be used for the quantitative evaluationof the kalopanaxsaponins of K. pictus.Key wordsKalopanx pictus, Araliaceae, saponin, kalopanaxsaponin, quantitative