0 8

Cited 0 times in

Polymerase chain reaction identification of microorganisms in previously root-filled teeth in a South Korean population

Authors
 Isabela N Rôças  ;  Il-Young Jung  ;  Chan-Young Lee  ;  José F Siqueira Jr 
Citation
 JOURNAL OF ENDODONTICS, Vol.30(7) : 504-508, 2004-07 
Journal Title
 JOURNAL OF ENDODONTICS 
ISSN
 0099-2399 
Issue Date
2004-07
MeSH
Adolescent ; Adult ; Aged ; Bacteria / classification* ; Bacteroides / isolation & purification ; Bacteroides Infections / microbiology ; DNA Primers ; Dental Pulp Cavity / microbiology* ; Enterococcus faecalis / isolation & purification ; Female ; Gram-Positive Bacterial Infections / microbiology ; Humans ; Korea ; Male ; Middle Aged ; Periapical Diseases / microbiology ; Polymerase Chain Reaction ; RNA, Bacterial / analysis ; RNA, Ribosomal, 16S / analysis ; Root Canal Therapy* ; Streptococcal Infections / microbiology ; Streptococcus / classification ; Treatment Failure
Abstract
A large body of evidence indicates that microorganisms are the primary causative agents of endodontic treatment failures. This study intended to assess the occurrence of nine putative endodontic pathogens in root-filled teeth associated with periradicular lesions in a South Korean population using a culture-independent molecular approach. Fourteen root-filled teeth with persistent periradicular diseases were selected for retreatment. After removal of the root canal filling, the canals were sampled, and a polymerase chain reaction assay using taxon-specific oligonucleotide primers was used for microbial detection. Bacteria were present in all cases, as revealed by amplification using ubiquitous 16S rDNA primers. The most frequently detected taxon was Enterococcus faecalis (64%), followed by Streptococcus spp. (21%) and Tannerella forsythensis (14%). The results of this study using a highly sensitive identification method are concurrent with those from other geographical locations using diverse identification methods in that E. faecalis is the main species found in cases of root-filled teeth associated with periradicular lesions.
Full Text
https://www.sciencedirect.com/science/article/pii/S0099239905602257
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
Yonsei Authors
Jung, Il Young(정일영) ORCID logo https://orcid.org/0000-0001-8972-2664
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178822
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links