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Nephroblastoma overexpressed gene (NOV) expression in rat hepatic stellate cells

Authors
 Sung Hee Lee  ;  Geom Seog Seo  ;  Young Nyun Park  ;  Dong Hwan Sohn 
Citation
 BIOCHEMICAL PHARMACOLOGY, Vol.68(7) : 1391-1400, 2004-10 
Journal Title
 BIOCHEMICAL PHARMACOLOGY 
ISSN
 0006-2952 
Issue Date
2004-10
MeSH
Animals ; Bile Acids and Salts / pharmacology ; Connective Tissue Growth Factor ; Dexamethasone / pharmacology ; Gene Expression* / drug effects ; Humans ; Immediate-Early Proteins / metabolism* ; Immunohistochemistry ; Intercellular Signaling Peptides and Proteins / metabolism* ; Liver / metabolism ; Liver Cirrhosis / metabolism ; Nephroblastoma Overexpressed Protein ; RNA, Messenger / drug effects ; RNA, Messenger / metabolism ; Rats ; Rats, Sprague-Dawley ; Stellate Ganglion / cytology* ; Stellate Ganglion / metabolism ; Transforming Growth Factor beta / pharmacology ; Transforming Growth Factor beta1
Abstract
Using the expression-profiling method, we identified nephroblastoma overexpressed gene (NOV) mRNA as one member of the mRNA population that was upregulated in cultured activated hepatic stellate cell (HSC). Northern analysis showed that NOV mRNA was increasingly expressed during progressive activation of cultured rat HSCs, and a significant increase was observed in both the carbon tetrachloride-induced and bile duct ligation/scission rat models of liver fibrosis. RT-PCR showed human NOV mRNA was increased in most fibrotic livers compared with normal livers. The expression of NOV protein in fibrotic rat and human livers was predominantly located in areas of ductular proliferation and HSC of the fibrous septa. HSCs stimulated with transforming growth factor beta1 showed increased expression of NOV protein without changing its mRNA levels. Dexamethasone stimulated the expression of NOV mRNA and protein. Furthermore, we demonstrated that bile acids have a modulating effect on the induction of NOV mRNA expression. In conclusion, this study suggests that NOV is expressed during liver fibrogenesis and HSCs may be an important source of hepatic NOV.
Full Text
https://www.sciencedirect.com/science/article/pii/S000629520400423X
DOI
10.1016/j.bcp.2004.06.009
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Nyun(박영년) ORCID logo https://orcid.org/0000-0003-0357-7967
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/178787
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