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Hyperosmotic Stimulus Down-regulates 1alpha, 25-dihydroxyvitamin D(3)-induced Osteoclastogenesis by Suppressing the RANKL Expression in a Co-culture System
DC Field | Value | Language |
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dc.contributor.author | 서정택 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 유윤정 | - |
dc.date.accessioned | 2020-08-14T03:15:41Z | - |
dc.date.available | 2020-08-14T03:15:41Z | - |
dc.date.issued | 2010-06 | - |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/178655 | - |
dc.description.abstract | The hyperosmotic stimulus is regarded as a mechanical factor for bone remodeling. However, whether the hyperosmotic stimulus affects 1alpha, 25-dihydroxyvitamin D(3) (1alpha,25(OH)(2)D(3))-induced osteoclastogenesis is not clear. In the present study, the effect of the hyperosmotic stimulus on 1alpha,25(OH)(2)D(3)-induced osteoclastogenesis was investigated in an osteoblast-preosteoclast co-culture system. Serial doses of sucrose were applied as a mechanical force. These hyperosmotic stimuli significantly evoked a reduced number of 1alpha,25(OH)(2)D(3)-induced tartrate-resistant acid phosphatase-positive multinucleated cells and 1alpha,25(OH)(2)D(3)-induced bone-resorbing pit area in a co-culture system. In osteoblastic cells, receptor activator of nuclear factor kappaB ligand (RANKL) and Runx2 expressions were down-regulated in response to 1alpha,25(OH)(2)D(3). Knockdown of Runx2 inhibited 1alpha,25(OH)(2)D(3)-induced RANKL expression in osteoblastic cells. Finally, the hyperosmotic stimulus induced the overexpression of TonEBP in osteoblastic cells. These results suggest that hyperosmolarity leads to the down-regulation of 1alpha,25(OH)(2)D(3)-induced osteoclastogenesis, suppressing Runx2 and RANKL expression due to the TonEBP overexpression in osteoblastic cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | 대한약리학회 | - |
dc.relation.isPartOf | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Hyperosmotic Stimulus Down-regulates 1alpha, 25-dihydroxyvitamin D(3)-induced Osteoclastogenesis by Suppressing the RANKL Expression in a Co-culture System | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학교실) | - |
dc.contributor.googleauthor | Yu Shun Tian | - |
dc.contributor.googleauthor | Hyun Joo Jeong | - |
dc.contributor.googleauthor | Sang-Do Lee | - |
dc.contributor.googleauthor | Seok Heui Kong | - |
dc.contributor.googleauthor | Seung-Ho Ohk | - |
dc.contributor.googleauthor | Yun-Jung Yoo | - |
dc.contributor.googleauthor | Jeong-Taeg Seo | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.contributor.googleauthor | Byung-Wha Sohn | - |
dc.contributor.googleauthor | Syng-Ill Lee | - |
dc.identifier.doi | 10.4196/kjpp.2010.14.3.169 | - |
dc.contributor.localId | A01905 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A02490 | - |
dc.relation.journalcode | J02104 | - |
dc.identifier.eissn | 2093-3827 | - |
dc.identifier.pmid | 20631890 | - |
dc.subject.keyword | Hyperosmotic stimulus | - |
dc.subject.keyword | Osteoblast | - |
dc.subject.keyword | RANKL | - |
dc.subject.keyword | Runx2 | - |
dc.subject.keyword | TonEBP | - |
dc.contributor.alternativeName | Seo, Jeong Taeg | - |
dc.contributor.affiliatedAuthor | 서정택 | - |
dc.contributor.affiliatedAuthor | 신동민 | - |
dc.contributor.affiliatedAuthor | 유윤정 | - |
dc.citation.volume | 14 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 169 | - |
dc.citation.endPage | 176 | - |
dc.identifier.bibliographicCitation | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, Vol.14(3) : 169-176, 2010-06 | - |
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