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ELISA를 이용한 자가항체 검사에 관한 연구

Other Titles
 Measurement of Autoantibodies by ELISA Kits 
 김현경  ;  김현숙  ;  권오헌  ;  이수곤 
 Korean Journal of Clinical Pathology (대한임상병리학회지), Vol.17(2) : 321-330, 1997 
Journal Title
 Korean Journal of Clinical Pathology (대한임상병리학회지) 
Issue Date
Background :The detection of specific autoantibodies in the sera of patients with systemic rheumatic diseases plays a key role in the differential diagnosis. Enzyme-linked immunosorbent assays (ELISA) is known as sensitive and semiquantitative method to detect autoantibodies and ELISA kits using a recombinant fusion protein as antigen have been developed. So, various commercial ELISA have recently become available in a diagnostic laboratory. We investigated the clinical value of antinuclear autoantibodies using commercial ELISA kits. Method :The serum of 90 patients were tested for autoantibodies to SSA/Ro, SSB/La, nRNP/Sm and Sm antigens by ELISA using four commercial kits, EL-ANA(TheraTest, IL, USA), DIASTAT(SHIELD, DUNDEE, UK), QUANTALite (INOVA, CA, USA), Y7relisa (alias, Wl, 7SA) We evaluated the clinical usefulness of panel test of Varelisa in the diagnosis of systemic rheumatic diseases. Result :The concordance rates of four ELISA kits for autoantibodies to SSA/Ro, SSB/La, RNP/Sm and Sm antigens were 83.6%, 74.5%, 87.5% and 80.0%, respectively. Using panel test of Varelisa, positive rates of autoantibodies to U1-snRNP, nRNP/Sm, Sm, SSA/Ro, SSB/La, Scl-70, CENP and Jo-1 antigens in SLE were 30.0, 40.0, 33.3, 46.7, 20.0, 20.0, 10.0, and 0%, respectively. Of 30 patients with SLE, 16 (53.3%) were positive for 2 or more antibodies. Conclusion :EL-ANA, QUANTALite and Varelisa show more positive rates than DIASTAT. The difference in the positive rates among four commercial ELISA kits may come from the different antigen sources. The panel test of 8 autoantibodies using Varelisa ELISA kit offers discriminative power and enhances the specificity of the assay in patients who lack clear evidence of clinically definite autoimmune disease.
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
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