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신장이식 前後에 측정한 각종 C형 간염바이러스 표식자 양성의 의미 -제3세대 ELISA 항체검사와 PCR법에 의한 RNA 검사를 중심으로 -

Other Titles
 Combined Interpretation of Pretransplant Anti - hepatitis C Virus ( HCV ) Antibody by 3rd Generation ELISA and HCV - RNA by Polymerase Chain Reaction ( PCR ) for the Prediction of Posttransplant Liver Dysfunction 
 김유선  ;  김명수  ;  이준호  ;  김현숙  ;  김순일  ;  문장일  ;  오소향  ;  이은미  ;  이호영  ;  박기일 
 Journal of the Korean Society for Transplantation (대한이식학회지), Vol.11(1) : 73-80, 1997 
Journal Title
 Journal of the Korean Society for Transplantation (대한이식학회지) 
Issue Date
Currently accurate test for identification of HCV infection is not yet developed. We examined 1) the relationship between the result of anti-HCV by 3rd generation ELISA (ELISA/3) and of HCV-RNA by PCR from pre-transplant stored serum in 89 living donor renal transplant recipients and 2) the correlation between the result of each or combined tests and the development of post-transplant liver dysfunction (LDF). LDF was defined as the increment of serum transaminase over 100 1.U./ml in two consecutive tests. Patients with climically or biopsy proven LDF by cyclosporine were excluded. Pre-transplant HCV infection rate sed by ELISA/3 and PCR was 20.2 and 29.2% respectively. Patients with PCR (+) developed LDF frequently comparesd with negative partners (50 vs. 27%, p=0.0367). Significant disparity between ELISA/3 and PCR was present. In 71 ELISA/3 (-) patients, 16(22.5%) were positive for PCR. However 8 (44.4%) were negative for PCR in 18 ELISA/3 (+) patients. ELISA/3 (+) or PCR (+) patients developed LDF frequently rather than ELISA/3 (-)/PCR(- ) ones(50.0 vs. 23.6%, p=0.0106). We could explain these data with S possibilities, 1) end-stage renal failure patients had a blunt antibody production, 2) serum sample may be collected during the window period for antibody formation, 3) even ELISA/3 could not detect fine molecular response during the early HCV infection(false negative), 4) significant false positive or serum contamination in PCR test, and finally 5) self-clearing of HCV antigen may be present in the body. In conclusion, HCV detection by PCR method and antibody test by ELISA/3 must be complementary for the accurate evaluation of HCV infection during the recipient evaluation and posttransplantation follow-up period.
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1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
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