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신장이식 前後에 측정한 각종 C형 간염바이러스 표식자 양성의 의미 -제3세대 ELISA 항체검사와 PCR법에 의한 RNA 검사를 중심으로 -

Other Titles
 Combined Interpretation of Pretransplant Anti - hepatitis C Virus ( HCV ) Antibody by 3rd Generation ELISA and HCV - RNA by Polymerase Chain Reaction ( PCR ) for the Prediction of Posttransplant Liver Dysfunction 
Authors
 김유선  ;  김명수  ;  이준호  ;  김현숙  ;  김순일  ;  문장일  ;  오소향  ;  이은미  ;  이호영  ;  박기일 
Citation
 Journal of the Korean Society for Transplantation (대한이식학회지), Vol.11(1) : 73-80, 1997 
Journal Title
 Journal of the Korean Society for Transplantation (대한이식학회지) 
ISSN
 1298-1711 
Issue Date
1997
Abstract
Currently accurate test for identification of HCV infection is not yet developed. We examined 1) the relationship between the result of anti-HCV by 3rd generation ELISA (ELISA/3) and of HCV-RNA by PCR from pre-transplant stored serum in 89 living donor renal transplant recipients and 2) the correlation between the result of each or combined tests and the development of post-transplant liver dysfunction (LDF). LDF was defined as the increment of serum transaminase over 100 1.U./ml in two consecutive tests. Patients with climically or biopsy proven LDF by cyclosporine were excluded. Pre-transplant HCV infection rate sed by ELISA/3 and PCR was 20.2 and 29.2% respectively. Patients with PCR (+) developed LDF frequently comparesd with negative partners (50 vs. 27%, p=0.0367). Significant disparity between ELISA/3 and PCR was present. In 71 ELISA/3 (-) patients, 16(22.5%) were positive for PCR. However 8 (44.4%) were negative for PCR in 18 ELISA/3 (+) patients. ELISA/3 (+) or PCR (+) patients developed LDF frequently rather than ELISA/3 (-)/PCR(- ) ones(50.0 vs. 23.6%, p=0.0106). We could explain these data with S possibilities, 1) end-stage renal failure patients had a blunt antibody production, 2) serum sample may be collected during the window period for antibody formation, 3) even ELISA/3 could not detect fine molecular response during the early HCV infection(false negative), 4) significant false positive or serum contamination in PCR test, and finally 5) self-clearing of HCV antigen may be present in the body. In conclusion, HCV detection by PCR method and antibody test by ELISA/3 must be complementary for the accurate evaluation of HCV infection during the recipient evaluation and posttransplantation follow-up period.
Full Text
http://kiss.kstudy.com/thesis/thesis-view.asp?key=2503003
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177818
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