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혈관내막세포 단층배양에서 동시배양 세포의 악성도에 따른 투과도 변화

Other Titles
 Permeability Changes of Capillary Endothelial Monolayer according to the Malignancy of Co-cultured Cell Lines 
 윤수한  ;  조기홍  ;  김세혁  ;  안영환  ;  안영민  ;  조경기  ;  최중언 
 Journal of Korean Neurosurgical Society (대한신경외과학회지), Vol.26(11) : 1493-1501, 1997 
Journal Title
 Journal of Korean Neurosurgical Society (대한신경외과학회지) 
Issue Date
Blood-brain barrier ; In vitro model ; Malignant brain tumor ; Peritumoral edema ; Transendothelial permeability ; Electrical resistanc
Even though many hypotheses have been derived from the anatomical and functional analysis of in vivo models of brain tumors, it is still impossible to explain the mechanism of peritumoral edema. To determine whether increased permeability in a blood-brain barrier model correlated with the malignancy of a co-cultured brain tumor, the authors established an in vitro brain capillary endothelial monolayer co-culture model. Water-soluble factors which might explain the pathogenetic mechanism of peritumoral edema in brain tumors were expected and observed. The benign cell co-culture model used co-cultured astrocytoma cell lines such as C6 and H683 in the second compartment of a brain capillary endothelial monolayer culture model circumscribed with a 0.4u sized porous membrane which permitted communication of the media but limited cell migration to another compartment, and the malignant cell co-culture model used co-cultured glioblastoma cell lines such as 87MG and 373MG. Permeability at molecular weight 373 increased in the astrocytoma and glioblastoma co-culture models to 150% and 240% respectively, of that in a normal astrocyte co-culture model. Permeability at this molecular weight also increased in the astrocytoma- and glioblastoma-conditioned medium culture models to 38% and 131%, respectively, of that in a normal astrocytoma-conditioned medium culture model. The observed result was that permeability of the endothelial monolayer increased in accordance with the malignancy of co-cultured cells in the system permitting-other than cell migration-media transfer only. The result suggested that some factor soluble in media secreted from co-cultured cells changes the permeability of the endothelial monolayer and could explain the pathogenetic mechanism of peritumoral edema in malignant brain tumors.
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1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Choi, Joong Uhn(최중언)
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