정상성상세포와 혈관내막세포를 공막 분리 동시배양으로 구축한 체외혈뇌장벽 모델

Abstract

There are three types of the in vitro blood-brain barrier models that are endothelial monolayer, astrocyte and endothelial monolayer co-culture, and benign glioma and endothelial monolayer co-culture. We made these three types of models and evaluated permeability ratio for four molecular weight(MW 373, 4400, 9300, 38900) and electrical resistance of endothelial monolayer in each type. Astrocyte and endothelial monolayer co-culture showed the lowest permeability ratio and highest electrical resistance among them and that suggested astrocyte and endothelial monolayer co-culture was best for the in-vitro blood-brain barrier model.