11 16

Cited 0 times in

Granzyme B immunoreactivity in T/natural killer cell lymphomas

Authors
 Min Sun Cho  ;  Mi Kyung Lee  ;  Woo Ick Yang  ;  Joo Heon Yoon 
Citation
 YONSEI MEDICAL JOURNAL, Vol.38(5) : 285-293, 1997 
Journal Title
 YONSEI MEDICAL JOURNAL 
ISSN
 0513-5796 
Issue Date
1997
MeSH
Adult ; Aged ; Aged, 80 and over ; Child ; Female ; Granzymes ; Humans ; Immunohistochemistry ; Killer Cells, Natural/pathology* ; Lymphoma/enzymology* ; Lymphoma/pathology* ; Lymphoma, T-Cell/enzymology* ; Lymphoma, T-Cell/pathology ; Male ; Middle Aged ; Serine Endopeptidases/metabolism*
Abstract
Granzyme B is one of the serine proteases expressed in natural killer (NK) cells and activated cytotoxic T-lymphocytes. To evaluate the usefulness of granzyme B immunoreactivity in the diagnosis of T/NK-cell lymphoma, we studied 69 cases of lymphomas occurring in the upper aerodigestive tract by paraffin-section immunohistochemistry using a commercially available monoclonal antibody to granzyme B (GrB-7). All 19 cases of T/NK-cell lymphomas defined by the expression of CD56 (NHK-1) and one or both T-cell markers (polyclonal CD3 and CD45RO) expressed granular cytoplasmic granzyme B immunoreactivity. Two out of 9 cases of T-cell lymphomas showing no CD56 expression demonstrated strong granzyme B immunoreactivity. No tumor cells among 39 cases of B-cell diffuse large cell lymphomas and 2 cases of null cell diffuse large cell lymphomas were immunoreactive for granzyme B, however a few scattered granzyme B-positive reactive small lymphoid cells were consistently observed. Based on its sensitivity in this study as well as its reactivity in routinely processed tissue sections, even without heat-induced epitope retrieval technique, monoclonal antibody to granzyme B (GrB-7) can be applied as a useful marker in the diagnosis of T/NK-cell lymphomas in conjunction with CD56.
Files in This Item:
T199702973.pdf Download
DOI
10.3349/ymj.1997.38.5.285
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Joo Heon(윤주헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177447
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links