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Monitoring of WT-1 gene expression in peripheral blood of patients with acute leukemia by semiquantitative RT-PCR; possible marker for detection of minimal residual leukemia

 Seong Cheol Kim  ;  Nae Choon Yoo  ;  Jee Sook Hahn  ;  Seok Lee  ;  So Young Chong  ;  Yoo Hong Min  ;  Yun Woong Ko 
 YONSEI MEDICAL JOURNAL, Vol.38(4) : 212-219, 1997 
Journal Title
Issue Date
Biomarkers, Tumor ; Gene Expression/physiology* ; Humans ; Leukemia, Myeloid, Acute/blood* ; Leukemia, Myeloid, Acute/genetics* ; Neoplasm, Residual ; Polymerase Chain Reaction ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/blood* ; Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics* ; Transcription, Genetic ; Wilms Tumor/genetics*
The expression of the WT-1 gene which is found exclusively in human leukemic blasts frequently disappears from bone marrow of leukemia patients in complete remission (CR). Using semiquantitative RT-PCR, we investigated the expression of the WT-1 gene in peripheral bloods (PBs) of 33 patients with acute leukemia (AML 26; ALL 7) and monitored its expression after achievement of CR. None of the 6 normal controls expressed detectable levels of WT-1 transcripts (< 10(-4), background level), whereas 31 (93.9%) of 33 patients expressed variable levels of WT-1 transcripts (range, 10(-4) to 10(1)) at diagnosis. The level of WT-1 expression was not different between AML and ALL. By monitoring WT-1 gene expression in PB of 31 patients during CR, 5 patients relapsed (two from the 18 patients with undetectable levels of WT-1 gene expression and three from the 13 with WT-1 gene expression in low levels). Three of the 5 relapsed patients showed preceding reappearance or rise of WT-1 gene expression. From these results, we reconfirmed that the WT-1 gene is a pan-acute leukemic marker, which can be used to monitor minimal residual leukemia (MRL) after chemotherapy or in patients with CR.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Min, Yoo Hong(민유홍) ORCID logo https://orcid.org/0000-0001-8542-9583
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