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Monitoring of WT-1 gene expression in peripheral blood of patients with acute leukemia by semiquantitative RT-PCR; possible marker for detection of minimal residual leukemia
DC Field | Value | Language |
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dc.contributor.author | 민유홍 | - |
dc.date.accessioned | 2020-07-03T17:18:39Z | - |
dc.date.available | 2020-07-03T17:18:39Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 0513-5796 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/177430 | - |
dc.description.abstract | The expression of the WT-1 gene which is found exclusively in human leukemic blasts frequently disappears from bone marrow of leukemia patients in complete remission (CR). Using semiquantitative RT-PCR, we investigated the expression of the WT-1 gene in peripheral bloods (PBs) of 33 patients with acute leukemia (AML 26; ALL 7) and monitored its expression after achievement of CR. None of the 6 normal controls expressed detectable levels of WT-1 transcripts (< 10(-4), background level), whereas 31 (93.9%) of 33 patients expressed variable levels of WT-1 transcripts (range, 10(-4) to 10(1)) at diagnosis. The level of WT-1 expression was not different between AML and ALL. By monitoring WT-1 gene expression in PB of 31 patients during CR, 5 patients relapsed (two from the 18 patients with undetectable levels of WT-1 gene expression and three from the 13 with WT-1 gene expression in low levels). Three of the 5 relapsed patients showed preceding reappearance or rise of WT-1 gene expression. From these results, we reconfirmed that the WT-1 gene is a pan-acute leukemic marker, which can be used to monitor minimal residual leukemia (MRL) after chemotherapy or in patients with CR. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | English | - |
dc.publisher | Yonsei University | - |
dc.relation.isPartOf | YONSEI MEDICAL JOURNAL | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Biomarkers, Tumor | - |
dc.subject.MESH | Gene Expression/physiology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Leukemia, Myeloid, Acute/blood* | - |
dc.subject.MESH | Leukemia, Myeloid, Acute/genetics* | - |
dc.subject.MESH | Neoplasm, Residual | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Precursor Cell Lymphoblastic Leukemia-Lymphoma/blood* | - |
dc.subject.MESH | Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics* | - |
dc.subject.MESH | Transcription, Genetic | - |
dc.subject.MESH | Wilms Tumor/genetics* | - |
dc.title | Monitoring of WT-1 gene expression in peripheral blood of patients with acute leukemia by semiquantitative RT-PCR; possible marker for detection of minimal residual leukemia | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Seong Cheol Kim | - |
dc.contributor.googleauthor | Nae Choon Yoo | - |
dc.contributor.googleauthor | Jee Sook Hahn | - |
dc.contributor.googleauthor | Seok Lee | - |
dc.contributor.googleauthor | So Young Chong | - |
dc.contributor.googleauthor | Yoo Hong Min | - |
dc.contributor.googleauthor | Yun Woong Ko | - |
dc.identifier.doi | 10.3349/ymj.1997.38.4.212 | - |
dc.contributor.localId | A01407 | - |
dc.relation.journalcode | J02813 | - |
dc.identifier.eissn | 1976-2437 | - |
dc.identifier.pmid | 9339129 | - |
dc.contributor.alternativeName | Min, Yoo Hong | - |
dc.contributor.affiliatedAuthor | 민유홍 | - |
dc.citation.volume | 38 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 212 | - |
dc.citation.endPage | 219 | - |
dc.identifier.bibliographicCitation | YONSEI MEDICAL JOURNAL, Vol.38(4) : 212-219, 1997 | - |
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