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Transforming growth factor-beta 1 responsiveness of human articular chondrocytes in vitro: normal versus osteoarthritis

Authors
 Jun Seop Jahng  ;  Jin Woo Lee  ;  Chang Dong Han  ;  Sung Jae Kim  ;  Nae Choon Yoo 
Citation
 YONSEI MEDICAL JOURNAL, Vol.38(1) : 40-51, 1997 
Journal Title
 YONSEI MEDICAL JOURNAL 
ISSN
 0513-5796 
Issue Date
1997
MeSH
Cartilage, Articular/cytology ; Cartilage, Articular/drug effects* ; Cell Division/drug effects ; Cells, Cultured ; Humans ; Osteoarthritis/pathology ; Reference Values ; Transforming Growth Factor beta/pharmacology*
Abstract
The transforming growth factor-β1 was known as having the most important influence on chondrocytes among various growth factors, being abundant in articular chondrocytes and osteocytes. We performed in vitro monolayer cultures of human articular chondrocytes from normal and osteoarthritic patients and studied the transforming growth factor-β1 responsiveness of those chondrocytes. The cell-growth curve indicated that the primary osteoarthritic chondrocyte culture with transforming growth factor-β1 showed a more rapid growth pattern than normal chondrocytes with or without TGF-β1 and osteoarthritic chondrocytes without TGF-β1. The osteoarthritic group showed a sharp decline in growth pattern with subsequent culture. The shape of osteoarthritic chondrocytes was bigger and more bizarre compared to those of normal chondrocytes. With subsequent culture, this change became prominent. The transforming growth factor-β1 increased the [3H]-TdR uptake in each group. The phenotypes of chondrocytes were more clearly expressed in the normal group. The chondrocytes lost their phenotype (production of collagen type II) following subculture in each group. The transforming growth factor-β1 could not inhibit or delay the dedifferentiation process (loss of phenotype).
Files in This Item:
T199702841.pdf Download
DOI
10.3349/ymj.1997.38.1.40
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sung Jae(김성재)
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177423
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