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Induction of Thermotolerance in Early Postimplantation Rat Embryos Is Associated With Increased Resistance to Hyperthermia-Induced Apoptosis

Authors
 PHILIP E. MIRKES  ;  L.M. CORNEL  ;  H.W. PARK  ;  M.L. CUNNINGHAM 
Citation
 TERATOLOGY, Vol.56(3) : 210-219, 1997 
Journal Title
 TERATOLOGY 
ISSN
 1542-0752 
Issue Date
1997
MeSH
Animals ; Apoptosis/physiology* ; Body Temperature Regulation/physiology* ; DNA Fragmentation/genetics ; Embryo, Mammalian/physiology* ; Embryonic Development/physiology* ; Eye/embryology ; Female ; Hot Temperature/adverse effects* ; Hyperthermia, Induced* ; In Vitro Techniques ; Kinetics ; Pregnancy ; Prosencephalon/embryology ; Rats ; Rats, Sprague-Dawley
Abstract
Previously we reported that hyperthermia (43 degrees C) induces cell death in neurulation stage rat embryos as part of the pathogenesis culminating in abnormal growth and development. We now show that hyperthermia-induced cell death occurs by a process termed apoptosis. DNA fragmentation, a hallmark of apoptosis, was noted as early as 2.5 hr after embryos were exposed to 43 degrees C. A smaller but significant increase in DNA fragmentation was also observed in embryos exposed to 42 degrees C, but only at the 5 hr time point. In control embryos, TUNEL-positive apoptotic bodies were consistently observed in the neuroepithelium at the point of neural tube closure and in the optic stalk. In embryos exposed to 43 degrees C, the number of TUNEL-positive apoptotic bodies was significantly increased. Using both gel electrophoresis and TUNEL, we also show that the induction of thermotolerance is associated with a significant reduction in DNA fragmentation. Together our results show that specific programmed cell death and hyperthermia-induced cell death correlate with internucleosomal DNA fragmentation characteristic of apoptosis. Finally, we show that the induction of thermotolerance in rat embryos is associated with a significant reduction in internucleosomal DNA fragmentation and associated apoptosis.
Full Text
https://onlinelibrary.wiley.com/doi/abs/10.1002/%28SICI%291096-9926%28199709%2956%3A3%3C210%3A%3AAID-TERA4%3E3.0.CO%3B2-4
DOI
10.1002/(SICI)1096-9926(199709)56:3<210::AID-TERA4>3.0.CO;2-4
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Park, Hyoung Woo(박형우) ORCID logo https://orcid.org/0000-0002-5341-9249
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177411
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