0 18

Cited 0 times in

Detection of Orientia tsutsugamushi DNA in individual trombiculids using polymerase chain reaction in Korea

Authors
 Han Il REE  ;  Woo Hyun CHANG  ;  Sunho KEE  ;  In Yong LEE  ;  Soung Hoo JEON 
Citation
 Medical Entomology and Zoology, Vol.48(3) : 197-209, 1997 
Journal Title
 Medical Entomology and Zoology 
ISSN
 0424-7086 
Issue Date
1997
Abstract
In October 1995,field rodents and insectivores were captured at 5 localities in Chollanam-do and 3 localities in Choongchongnam-do, and a total of 81 field mice and shrews, including 64 Apodemus agrarius mice, were collected. The antibody positive rate of A. agrarius sera to Orientia tsutsugamushi was 52.4% in average. A total of 6,372 chigger mites were collected from the mice, and 10 species of 4 genera were identified. Leptotrombidium pallidum was predominant in Choong-chongnam-do, whereas L. scutellare was predominant in Chollanam-do. The chigger mites were individually dissected and internal contents were examined for O. tsutsugamushi infection applying IFA and PCR methods. Of 4,315 chigger mites screened by IFA, 115 positive and suspected cases were confirmed by consensus PCR. O. tsutsugamushi was found in 65 chiggers of 5 species, showing 1.51% infection rate in total. The infection rate of each 5 species was 1.64% in L. pallidum, 1.37% in L. scutellare, 1.77% in L. palpale, 1.60% in L. orientale and 1.41% in L. zetum. The detection of O. tsutsugamushi in L. orientale and L. zetum is the first finding in the world. The serotype of O. tsutsugamushi were also studied by analyzing the nucleotide sequences of the strain-specific region of 56 kDa protein, and all of the eight cases were of the F756-like type. This new sero-type was found from 4 species of Leptotrombidium, L. pallidum, L. scutellare, L. palpale and L. orientale.
DOI
10.7601/mez.48.197
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Ree, Han Il(이한일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177377
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links