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Detection of Orientia tsutsugamushi DNA in individual trombiculids using polymerase chain reaction in Korea

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dc.description.abstractIn October 1995,field rodents and insectivores were captured at 5 localities in Chollanam-do and 3 localities in Choongchongnam-do, and a total of 81 field mice and shrews, including 64 Apodemus agrarius mice, were collected. The antibody positive rate of A. agrarius sera to Orientia tsutsugamushi was 52.4% in average. A total of 6,372 chigger mites were collected from the mice, and 10 species of 4 genera were identified. Leptotrombidium pallidum was predominant in Choong-chongnam-do, whereas L. scutellare was predominant in Chollanam-do. The chigger mites were individually dissected and internal contents were examined for O. tsutsugamushi infection applying IFA and PCR methods. Of 4,315 chigger mites screened by IFA, 115 positive and suspected cases were confirmed by consensus PCR. O. tsutsugamushi was found in 65 chiggers of 5 species, showing 1.51% infection rate in total. The infection rate of each 5 species was 1.64% in L. pallidum, 1.37% in L. scutellare, 1.77% in L. palpale, 1.60% in L. orientale and 1.41% in L. zetum. The detection of O. tsutsugamushi in L. orientale and L. zetum is the first finding in the world. The serotype of O. tsutsugamushi were also studied by analyzing the nucleotide sequences of the strain-specific region of 56 kDa protein, and all of the eight cases were of the F756-like type. This new sero-type was found from 4 species of Leptotrombidium, L. pallidum, L. scutellare, L. palpale and L. orientale.-
dc.languageJapanese, English-
dc.publisherThe Japan Society of Medical Entomology and Zoology-
dc.relation.isPartOfMedical Entomology and Zoology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleDetection of Orientia tsutsugamushi DNA in individual trombiculids using polymerase chain reaction in Korea-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학교실)-
dc.contributor.googleauthorHan Il REE-
dc.contributor.googleauthorWoo Hyun CHANG-
dc.contributor.googleauthorSunho KEE-
dc.contributor.googleauthorIn Yong LEE-
dc.contributor.googleauthorSoung Hoo JEON-
dc.contributor.alternativeNameRee, Han Il-
dc.identifier.bibliographicCitationMedical Entomology and Zoology, Vol.48(3) : 197-209, 1997-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers


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