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Correlation between K-ras gene mutation and prognosis of patients with nonsmall cell lung carcinoma

 Jae Yong Cho  ;  Joo Hang Kim  ;  Yi Hyeong Lee  ;  Kyung Young Chung  ;  Sung Kyu Kim  ;  Soo Jung Gong  ;  Nae Choon You  ;  Hyun Cheol Chung  ;  Jae Kyung Roh  ;  Byung Soo Kim 
 CANCER, Vol.79(3) : 462-467, 1997 
Journal Title
Issue Date
Adult ; Aged ; Carcinoma, Non-Small-Cell Lung/genetics* ; DNA Probes ; Disease Progression ; Female ; Genes, ras/genetics* ; Humans ; Lung Neoplasms/genetics* ; Male ; Middle Aged ; Mutation* ; Polymerase Chain Reaction ; Polymorphism, Single-Stranded Conformational ; Prognosis ; Survival Analysis
BACKGROUND: Mutations at codons 12, 13, and 61 of the three ras genes, H-ras, K-ras, and N-ras, convert these genes into active oncogenes. It appears that ras gene mutations can be found in a variety of tumor types. The purpose of this study was to evaluate the clinical significance of K-ras gene mutation in nonsmall cell lung carcinoma (NSCLC). METHODS: The authors analyzed 58 NSCLC patients for mutations at codons 12, 13, and 61 of the K-ras gene and correlated the findings with the tumor stage and patient survival. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and the direct nucleotide sequencing method were used to detect mutations after amplification of ras specific sequences by PCR. RESULTS: Fourteen mutations (24%) of ras genes were found, all at codon 12 of the K-ras gene. GGT to GAT transition was the predominant mutational pattern. There was a significant association between K-ras mutation and the tumor stage (i.e., the higher the stage, the higher the mutation rate) (P = 0.014). Using univariate analysis, the presence of K-ras mutation in paraffin embedded tissue from patients who received treatment with curative intent was associated with a shorter survival (P = 0.039). The median survival duration for patients with or without K-ras mutation was 9 and 30 months, respectively. The Cox proportional hazards model also predicted a higher risk for patients with K-ras mutations (P = 0.047). CONCLUSIONS: K-ras mutations, present in a subset of NSCLC, are associated with tumor progression and shortened patient survival.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Joo Hang(김주항)
Roh, Jae Kyung(노재경)
Chung, Kyung Young(정경영)
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
Cho, Jae Yong(조재용) ORCID logo https://orcid.org/0000-0002-0926-1819
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