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307-bp fragment in HOXA7 upstream sequence is sufficient for anterior boundary formation

Authors
 WONGI MIN  ;  HEE-JONG WOO  ;  CHUL-SANG LEE  ;  KYUNG-KWANG LEE  ;  WON KEE YOON  ;  HYOUNG WOO PARK  ;  MYOUNG HEE KIM 
Citation
 DNA AND CELL BIOLOGY, Vol.17(3) : 293-299, 1998 
Journal Title
DNA AND CELL BIOLOGY
ISSN
 1044-5498 
Issue Date
1998
MeSH
Animals ; Central Nervous System/embryology ; Drosophila Proteins ; Female ; Ganglia, Spinal/embryology ; Gene Expression Regulation, Developmental* ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism ; Homeodomain Proteins/physiology* ; Humans ; Limb Buds ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Inbred Strains ; Mice, Transgenic ; Regulatory Sequences, Nucleic Acid* ; Sequence Deletion ; Transcription Factors ; beta-Galactosidase/genetics
Abstract
The HOX genes are expressed in a positionally and temporally restricted manner involving anteroposterior axial pattern formation during early embryogenesis. Previously, we studied the sequence and function of an upstream regulatory region of the human HOXA7 gene. To identify a critical cis-acting element, a deletion analysis was performed along the human control region (HCR) (about 1.1 kb), which was sufficient for setting the anterior boundary of expression in transgenic mice. We demonstrated that a 307-bp control region contains a cis-acting element(s) specifying an anterior boundary as well as a dorsal-ventral restriction in the neural tube at day 12.5 postconception (p.c.). The distinct anterior limit of expression was noted at the level of C7/T1 in the neural tube and spinal ganglia. In addition, our deletion experiments revealed that the HCR consisted of several cis-acting elements which were individually capable of driving regionally restricted expression patterns in the neural tube and limb buds.
Full Text
https://www.liebertpub.com/doi/abs/10.1089/dna.1998.17.293
DOI
10.1089/dna.1998.17.293
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
Park, Hyoung Woo(박형우) ORCID logo https://orcid.org/0000-0002-5341-9249
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/177198
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