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Synergy between Entamoeba histolytica and Escherichia coli in the induction of cytokine gene expression in human colon epithelial cells

Authors
 Jung Mogg Kim  ;  Hyun Chae Jung  ;  Kyung-il Im  ;  In Sung Song  ;  Chung Yong Kim 
Citation
 PARASITOLOGY RESEARCH, Vol.84(0) : 509-512, 1998 
Journal Title
 PARASITOLOGY RESEARCH 
ISSN
 0932-0113 
Issue Date
1998
MeSH
Animals ; Coculture Techniques ; Colon/metabolism* ; Colon/microbiology ; Colon/parasitology ; Entamoeba histolytica/physiology* ; Epithelial Cells/metabolism ; Epithelial Cells/microbiology ; Epithelial Cells/parasitology ; Escherichia coli/physiology* ; Gene Expression Regulation* ; Granulocyte-Macrophage Colony-Stimulating Factor/genetics ; Growth Substances/genetics* ; HT29 Cells ; Humans ; Interleukin-1/genetics ; Interleukin-8/genetics ; Lipopolysaccharides/pharmacology ; RNA, Messenger/analysis ; RNA, Messenger/genetics ; Up-Regulation
Abstract
Entamoeba histolytica induces the expression and secretion of interleukin 8 (IL-8) by cultured colon epithelial cells. We assessed the array of cytokine genes expressed by human colon epithelial cells in response to co-culture with E. histolytica trophozoites and tested the hypothesis that enteric bacteria may alter the E. histolytica-induced expression of such genes. HT-29 colon epithelial cells were co-cultured with E. histolytica trophozoites in the presence or absence of Escherichia coli. Cytokine gene expression was assessed by quantitative reverse-transcription polymerase chain reaction (RT-PCR). IL-8 mRNA in colon epithelial cells was up-regulated following exposure to E. histolytica and this was paralleled by increased IL-8 secretion. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-1alpha/beta mRNAs were also up-regulated in these cells. When HT-29 cells were co-cultured with E. coli DH5alpha and E. histolytica there was a synergistic increase in the expression of IL-8, IL-1alpha, and GM-CSF. These results suggest that enteric bacteria may significantly affect early proinflammatory signals produced in host tissues in response to E. histolytica infection.
Full Text
https://link.springer.com/article/10.1007%2FBF03356595
DOI
10.1007/bf03356595
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Im, Kyung Il(임경일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/176625
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