0 295

Cited 16 times in

Synergy between Entamoeba histolytica and Escherichia coli in the induction of cytokine gene expression in human colon epithelial cells

DC Field Value Language
dc.contributor.author임경일-
dc.date.accessioned2020-07-02T17:09:05Z-
dc.date.available2020-07-02T17:09:05Z-
dc.date.issued1998-
dc.identifier.issn0932-0113-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/176625-
dc.description.abstractEntamoeba histolytica induces the expression and secretion of interleukin 8 (IL-8) by cultured colon epithelial cells. We assessed the array of cytokine genes expressed by human colon epithelial cells in response to co-culture with E. histolytica trophozoites and tested the hypothesis that enteric bacteria may alter the E. histolytica-induced expression of such genes. HT-29 colon epithelial cells were co-cultured with E. histolytica trophozoites in the presence or absence of Escherichia coli. Cytokine gene expression was assessed by quantitative reverse-transcription polymerase chain reaction (RT-PCR). IL-8 mRNA in colon epithelial cells was up-regulated following exposure to E. histolytica and this was paralleled by increased IL-8 secretion. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-1alpha/beta mRNAs were also up-regulated in these cells. When HT-29 cells were co-cultured with E. coli DH5alpha and E. histolytica there was a synergistic increase in the expression of IL-8, IL-1alpha, and GM-CSF. These results suggest that enteric bacteria may significantly affect early proinflammatory signals produced in host tissues in response to E. histolytica infection.-
dc.description.statementOfResponsibilityrestriction-
dc.languageEnglish-
dc.publisherSpringer International-
dc.relation.isPartOfPARASITOLOGY RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnimals-
dc.subject.MESHCoculture Techniques-
dc.subject.MESHColon/metabolism*-
dc.subject.MESHColon/microbiology-
dc.subject.MESHColon/parasitology-
dc.subject.MESHEntamoeba histolytica/physiology*-
dc.subject.MESHEpithelial Cells/metabolism-
dc.subject.MESHEpithelial Cells/microbiology-
dc.subject.MESHEpithelial Cells/parasitology-
dc.subject.MESHEscherichia coli/physiology*-
dc.subject.MESHGene Expression Regulation*-
dc.subject.MESHGranulocyte-Macrophage Colony-Stimulating Factor/genetics-
dc.subject.MESHGrowth Substances/genetics*-
dc.subject.MESHHT29 Cells-
dc.subject.MESHHumans-
dc.subject.MESHInterleukin-1/genetics-
dc.subject.MESHInterleukin-8/genetics-
dc.subject.MESHLipopolysaccharides/pharmacology-
dc.subject.MESHRNA, Messenger/analysis-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHUp-Regulation-
dc.titleSynergy between Entamoeba histolytica and Escherichia coli in the induction of cytokine gene expression in human colon epithelial cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Tropica Medicine (열대의학교실)-
dc.contributor.googleauthorJung Mogg Kim-
dc.contributor.googleauthorHyun Chae Jung-
dc.contributor.googleauthorKyung-il Im-
dc.contributor.googleauthorIn Sung Song-
dc.contributor.googleauthorChung Yong Kim-
dc.identifier.doi10.1007/bf03356595-
dc.contributor.localIdA03355-
dc.relation.journalcodeJ02467-
dc.identifier.eissn1432-1955-
dc.identifier.pmid9660143-
dc.identifier.urlhttps://link.springer.com/article/10.1007%2FBF03356595-
dc.contributor.alternativeNameIm, Kyung Il-
dc.contributor.affiliatedAuthor임경일-
dc.citation.volume84-
dc.citation.number0-
dc.citation.startPage509-
dc.citation.endPage512-
dc.identifier.bibliographicCitationPARASITOLOGY RESEARCH, Vol.84(0) : 509-512, 1998-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.