Cited 16 times in
Synergy between Entamoeba histolytica and Escherichia coli in the induction of cytokine gene expression in human colon epithelial cells
DC Field | Value | Language |
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dc.contributor.author | 임경일 | - |
dc.date.accessioned | 2020-07-02T17:09:05Z | - |
dc.date.available | 2020-07-02T17:09:05Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0932-0113 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/176625 | - |
dc.description.abstract | Entamoeba histolytica induces the expression and secretion of interleukin 8 (IL-8) by cultured colon epithelial cells. We assessed the array of cytokine genes expressed by human colon epithelial cells in response to co-culture with E. histolytica trophozoites and tested the hypothesis that enteric bacteria may alter the E. histolytica-induced expression of such genes. HT-29 colon epithelial cells were co-cultured with E. histolytica trophozoites in the presence or absence of Escherichia coli. Cytokine gene expression was assessed by quantitative reverse-transcription polymerase chain reaction (RT-PCR). IL-8 mRNA in colon epithelial cells was up-regulated following exposure to E. histolytica and this was paralleled by increased IL-8 secretion. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-1alpha/beta mRNAs were also up-regulated in these cells. When HT-29 cells were co-cultured with E. coli DH5alpha and E. histolytica there was a synergistic increase in the expression of IL-8, IL-1alpha, and GM-CSF. These results suggest that enteric bacteria may significantly affect early proinflammatory signals produced in host tissues in response to E. histolytica infection. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | Springer International | - |
dc.relation.isPartOf | PARASITOLOGY RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Coculture Techniques | - |
dc.subject.MESH | Colon/metabolism* | - |
dc.subject.MESH | Colon/microbiology | - |
dc.subject.MESH | Colon/parasitology | - |
dc.subject.MESH | Entamoeba histolytica/physiology* | - |
dc.subject.MESH | Epithelial Cells/metabolism | - |
dc.subject.MESH | Epithelial Cells/microbiology | - |
dc.subject.MESH | Epithelial Cells/parasitology | - |
dc.subject.MESH | Escherichia coli/physiology* | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | Granulocyte-Macrophage Colony-Stimulating Factor/genetics | - |
dc.subject.MESH | Growth Substances/genetics* | - |
dc.subject.MESH | HT29 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-1/genetics | - |
dc.subject.MESH | Interleukin-8/genetics | - |
dc.subject.MESH | Lipopolysaccharides/pharmacology | - |
dc.subject.MESH | RNA, Messenger/analysis | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | Up-Regulation | - |
dc.title | Synergy between Entamoeba histolytica and Escherichia coli in the induction of cytokine gene expression in human colon epithelial cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Tropica Medicine (열대의학교실) | - |
dc.contributor.googleauthor | Jung Mogg Kim | - |
dc.contributor.googleauthor | Hyun Chae Jung | - |
dc.contributor.googleauthor | Kyung-il Im | - |
dc.contributor.googleauthor | In Sung Song | - |
dc.contributor.googleauthor | Chung Yong Kim | - |
dc.identifier.doi | 10.1007/bf03356595 | - |
dc.contributor.localId | A03355 | - |
dc.relation.journalcode | J02467 | - |
dc.identifier.eissn | 1432-1955 | - |
dc.identifier.pmid | 9660143 | - |
dc.identifier.url | https://link.springer.com/article/10.1007%2FBF03356595 | - |
dc.contributor.alternativeName | Im, Kyung Il | - |
dc.contributor.affiliatedAuthor | 임경일 | - |
dc.citation.volume | 84 | - |
dc.citation.number | 0 | - |
dc.citation.startPage | 509 | - |
dc.citation.endPage | 512 | - |
dc.identifier.bibliographicCitation | PARASITOLOGY RESEARCH, Vol.84(0) : 509-512, 1998 | - |
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