Background :The TrepanostikaTM is an enzyme-linked immunosorbent assay (ELISA) for the qualitative determination of specific antibody (Ab) to Treponema pallidum. It is important to detect Treponenia pallidum-specific Ab to confirm syphilis in patients with positive nontreponemal result or at late latent stage/late stage. Currently various ELISA methods for Treponema pallidum-specific Ab haute been developed in addition to widely used treponemal tests such as fluorescent treponemal antibody absorption (FTA-ABS) test or Treponema pallidum hemagglutination (TPHA) test. We evaluated TrepanostikaTM, anti-treponemal ELISA test. Method :The sensitivity anti specificity of this ELISA method for detecting Treponema pallidum-specific Ab (TrepanostikaTM) were evaluated and compared with other treponemal tests such as FTA-ABS and TPHA. Result :The sensitivity and specificity of TrepanostikaTM were 55.7% and 95.8%, respectively. The concordance rate with FTA-ABS was 98.9% (283/286) and 100% (272/272) with TPHA. Conclusion :TrepanostikaTM which has similar sensitivity and specificity with FTA-ABS or TPHA could replace the well-known treponemal test such as FTA-ABS or TPHA.