0 15

Cited 0 times in

Farnesylcysteine methyltransferase activity and Ras protein expression in human stomach tumor tissue

Authors
 Eui-Sik Han  ;  Hye-Young Oh  ;  Kwang-Won Ha  ;  Beom-Seok Han  ;  Seok-Min Hong  ;  Jung-Whwan Han  ;  Sungyoul Hong  ;  Sung Hun Noh  ;  Hyang Woo Lee 
Citation
 ARCHIVES OF PHARMACAL RESEARCH, Vol.21(4) : 378-384, 1998 
Journal Title
 ARCHIVES OF PHARMACAL RESEARCH 
ISSN
 0253-6269 
Issue Date
1998
MeSH
Adenocarcinoma/enzymology ; Adenocarcinoma/metabolism ; Adenocarcinoma/pathology ; Adult ; Aged ; Cysteine/analogs & derivatives* ; Cysteine/metabolism ; Enzyme Activation/drug effects ; Gene Expression ; Humans ; Immunohistochemistry ; Middle Aged ; Protein Methyltransferases/antagonists & inhibitors ; Protein Methyltransferases/metabolism* ; S-Adenosylhomocysteine/pharmacology ; Stomach/enzymology ; Stomach Neoplasms/enzymology* ; Stomach Neoplasms/metabolism ; Stomach Neoplasms/pathology ; ras Proteins/analysis ; ras Proteins/biosynthesis* ; ras Proteins/metabolism
Abstract
The processing pathway of G-proteins and Ras family proteins includes the isoprenylation of the cysteine residue, followed by proteolysis of three terminal residues and alpha-carboxyl methyl esterification of the cysteine residue. Farnesylcysteine methyltransferase (FCMT) activity is responsible for the methylation reaction which play a role in the membrane attachment of a variety of cellular proteins. Four kinds of Ras protein (c-Ha-ras, c-N-Ras, c-Ki-Ras, pan-Ras) expression were detected in adenocarcinoma of human tissue by immunohistochemical method, and hematoxylin and eosin staining. The level of Ras protein in human stomach tumor tissues was much higher than in normal and peritumoral regions of the same biopsy samples. The FCMT activities of each cellular fractions were high in mitochondrial fraction followed by microsomal fraction, whole homogenate and cytosolic fraction. The inhibitory effect on FCMT activity on stomach tumor tissue was determined after treatment with 0.25 microM of S-adenosyl-L-homocysteine. S-adenosyl-L-homocysteine inhibited FCMT activity from 11.2% to 30.5%. These results suggested that FCMT might be involved in Ras proteins activity.
Full Text
https://link.springer.com/article/10.1007/bf02974630
DOI
10.1007/bf02974630
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Noh, Sung Hoon(노성훈) ORCID logo https://orcid.org/0000-0003-4386-6886
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/176503
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links