0 34

Cited 0 times in

Moxifloxacin Labeling-Based Multiphoton Microscopy of Skin Cancers in Asians

Authors
 Hoonchul Chang  ;  Won Hyuk Jang  ;  Seunghun Lee  ;  Bumju Kim  ;  Myoung Joon Kim  ;  Won Oh Kim  ;  Young Wook Ryoo  ;  Byung Ho Oh  ;  Ki Hean Kim 
Citation
 LASERS IN SURGERY AND MEDICINE, Vol.52(4) : 373-382, 2020-04 
Journal Title
 LASERS IN SURGERY AND MEDICINE 
ISSN
 0196-8092 
Issue Date
2020-04
Keywords
basal cell carcinoma ; high-speed imaging ; moxifloxacin ; multiphoton microscopy ; skin cancer
Abstract
Background and objectives: Although multiphoton microscopy (MPM) can visualize both cell and extracellular matrix (ECM) structures of the skin in high-contrast without exogenous labeling, label-free MPM is usually too slow to image clinically relevant large regions. A high-speed MPM method would be beneficial for evaluating clinical skin specimens by increasing the imaging area. In this study, moxifloxacin labeling-based MPM (moxifloxacin MPM) was characterized in various human skin cancer specimens. Study design/materials and methods: Moxifloxacin ophthalmic solution was used for cell-labeling and MPM imaging was conducted afterwards. Moxifloxacin MPM was characterized in ex vivo normal human skin and skin cancer specimens in comparison with the label-free MPM and fluorescence confocal microscopy (FCM) using acridine orange as a labeling agent. Then, moxifloxacin MPM was applied to various ex vivo human skin cancer specimens including basal cell carcinoma (BCC), squamous cell carcinoma (SCC), dermatofibrosarcoma protuberans (DFSP). Results of moxifloxacin MPM were compared with bright-field clinical and histopathologic findings. Results: Moxifloxacin MPM imaged both cells and collagen in the skin, similarly to label-free MPM, but with enhanced fluorescence intensities in cells and enhanced imaging speeds. Moxifloxacin MPM imaged cells in the skin similarly to acridine orange-based FCM. Moxifloxacin MPM of various human skin cancer specimens imaged their specific cellular features. The microscopic features detected in moxifloxacin MPM were confirmed with histological images. Conclusions: This observational pilot study demonstrated that moxifloxacin MPM could detect specific cellular features of various skin cancers in good correlation with histopathological images in Asian patients at the higher imaging speed than label-free MPM.
Full Text
https://onlinelibrary.wiley.com/doi/full/10.1002/lsm.23138
DOI
10.1002/lsm.23138
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Oh, Byung Ho(오병호) ORCID logo https://orcid.org/0000-0001-9575-5665
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/176106
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links