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Lactobacillus plantarum CBT LP3 ameliorates colitis via modulating T cells in mice

Authors
 Da Hye Kim  ;  Soochan Kim  ;  Jae Bum Ahn  ;  Jae Hyeon Kim  ;  Hyun Woo Ma  ;  Dong Hyuk Seo  ;  Xiumei Che  ;  Ki Cheong Park  ;  Jeon Jeong Yong  ;  Sang Yong Kim  ;  Han Cheol Lee  ;  Jae-Young Lee  ;  Tae Il Kim  ;  Won Ho Kim  ;  Seung Won Kim  ;  Jae Hee Cheon 
Citation
 INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, Vol.310(2) : e151391, 2020 
Journal Title
 INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY 
ISSN
 1438-4221 
Issue Date
2020
Keywords
Goblet cell ; Inflammatory bowel disease ; Lactobacillus plantarum CBT LP3 ; Proinflammatory cytokine ; T cell
Abstract
Lactobacillus plantarum has been identified as a probiotic bacterium owing to its role in immune regulation and maintenance of intestinal permeability. Here, we investigated the anti-colitic effects and mechanism of L. plantarum CBT LP3 (LP3). This in vivo study was performed using dextran sodium sulfate (DSS) to induce colitis in mice. Mice were randomly divided into three groups: a control supplied with normal drinking water, a DSS-treated group followed by oral administration of vehicle, and a DSS-treated group gavaged with LP3 daily for 7 days following DSS administration. An analysis of macrophages and T cell subsets harvesting from peritonium cavity cells and splenocytes was performed using a flow cytometric assay. Gene expression and cytokine profiles were measured using quantitative reverse transcriptase polymerase chain reaction. The administration of LP3 significantly attenuated disease activity and histolopathology compared to control. LP3 had anti-inflammatory effects, with increased induction of regulatory T cells and type 2 helper T cells in splenocytes and restoration of goblet cells accompanied by suppression of proinflammatory cytokine expressions. These findings suggest that L. plantarum CBT LP3 can be used as a potent immunomodulator, which has significant implications for IBD treatment.
Files in This Item:
T202000536.pdf Download
DOI
10.1016/j.ijmm.2020.151391
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Nuclear Medicine (핵의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Seung Won(김승원) ORCID logo https://orcid.org/0000-0002-1692-1192
Kim, Won Ho(김원호) ORCID logo https://orcid.org/0000-0002-5682-9972
Kim, Tae Il(김태일) ORCID logo https://orcid.org/0000-0003-4807-890X
Park, Ki Cheong(박기청) ORCID logo https://orcid.org/0000-0002-3435-3985
Jeon, Jeong Yong(전정용)
Cheon, Jae Hee(천재희) ORCID logo https://orcid.org/0000-0002-2282-8904
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/175531
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