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Increased Innate Lymphoid Cell 3 and IL-17 Production in Mouse Lamina Propria Stimulated with Giardia lamblia

Authors
 Hye-Yeon Lee  ;  Eun-Ah Park  ;  Kyung-Jo Lee  ;  Kyu-Ho Lee  ;  Soon-Jung Park 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.57(3) : 225-232, 2019 
Journal Title
 KOREAN JOURNAL OF PARASITOLOGY 
ISSN
 0023-4001 
Issue Date
2019
MeSH
Animals ; Cells, Cultured ; Giardia lamblia/physiology* ; Giardiasis/genetics ; Giardiasis/immunology* ; Giardiasis/parasitology ; Humans ; Immunity, Innate ; Interleukin-17/genetics ; Interleukin-17/immunology* ; Lymphocytes/immunology* ; Lymphocytes/parasitology ; Mice ; Mice, Inbred C57BL ; Mucous Membrane/immunology ; Mucous Membrane/parasitology*
Keywords
Giardia lamblia ; group 3 ; innate lymphoid cell ; interleukin-17
Abstract
Innate lymphoid cells (ILCs) are key players during an immune response at the mucosal surfaces, such as lung, skin, and gastrointestinal tract. Giardia lamblia is an extracellular protozoan pathogen that inhabits the human small intestine. In this study, ILCs prepared from the lamina propria of mouse small intestine were incubated with G. lamblia trophozoites. Transcriptional changes in G. lamblia-exposed ILCs resulted in identification of activation of several immune pathways. Secretion of interleukin (IL)-17A, IL-17F, IL-1β, and interferon-γ was increased, whereas levels of IL-13, IL-5, and IL22, was maintained or reduced upon exposure to G. lamblia. Goup 3 ILC (ILC3) was found to be dominant amongst the ILCs, and increased significantly upon co-cultivation with G. lamblia trophozoites. Oral inoculation of G. lamblia trophozoites into mice resulted in their presence in the small intestine, of which, the highest number of parasites was detected at the 5 days-post infection. Increased ILC3 was observed amongst the ILC population at the 5 days-post infection. These findings indicate that ILC3 from the lamina propria secretes IL-17 in response to G. lamblia, leading to the intestinal pathology observed in giardiasis.
Files in This Item:
T201905841.pdf Download
DOI
10.3347/kjp.2019.57.3.225
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/175219
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