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Chemokine in inflamed periodontal tissues activates healthy periodontal-ligament stem cell migration

Other Titles
 염증성 치주조직에서 유래된 케모카인에 의한 건강한 치주인대 줄기세포의 이동활성 증진에 관한 연구 
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Aim: The present study aimed to characterize the expression pattern of chemokines obtained from inflamed periodontal defects and to determine the characteristics of human periodontal-ligament stem cells (hPDLSCs) migrated by each specific chemokine. Materials and methods: Both inflamed and healthy periodontal tissues were obtained from periodontitis patients (n=11), and the chemokine expression levels were analyzed. The periodontal-tissue-specific chemokines were applied to healthy hPDLSCs from extracted teeth (n=3), with FGF-2 acting as a positive control. Cells were separated by selected chemokines using transwell method into migrated/unmigrated hPDLSCs. The characteristics of the hPDLSC subpopulation recruited by each chemokine were assessed, and gene expression pattern was analyzed by microarray. Results: Chemokines were categorized into three groups by specific patterns of ‘appearing’, ‘increasing’, and ‘decreasing/disappearing’ from healthy to inflamed tissues. A representative chemokine from each group enhanced the capacities for colony formation and osteogenic/adipogenic differentiation while maintaining the surface markers of hPDLSCs. RANTES/CCL5 significantly increased the cellular migration of hPDLSCs, via enhancement of signaling pathways, regulation of the actin skeleton, and focal adhesion. Conclusion: The present study found a specific chemokine profile induced by inflammation in periodontal tissues, with RANTES/CCL5 appearing to play a role in the migration of hPDLSCs into inflammatory periodontal lesions.
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2. College of Dentistry (치과대학) > Others (기타) > 5. Others
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