0 100

Cited 1 times in

Fluorescence fingerprints of oral bacteria

Authors
 Si‐Mook Kang  ;  Elbert de Josselin de Jong  ;  Susan M. Higham  ;  Christopher K. Hope  ;  Baek‐Il Kim 
Citation
 JOURNAL OF BIOPHOTONICS, Vol.13(1) : e201900190, 2020 
Journal Title
 JOURNAL OF BIOPHOTONICS 
ISSN
 1864-063X 
Issue Date
2020
Keywords
autofluorescence ; biophotonics ; identification ; imaging ; oral bacteria
Abstract
The rapid detection and identification of microorganisms is one of the most important factors in many cases of ill health. The purpose of this study was to determine the fluorescence characteristics of seven oral bacteria using emission spectra with the aim of distinguishing between the bacteria, and to compare fluorescence imaging methods for the direct assessment of oral bacteria. Fluorescence images of each bacterium were obtained under a 405-nm light source using a two-filter system. The emissions of all samples were measured with a fluorescence spectrometer. The complete fluorescence data set collected for each sample employed a three-dimensional data cube. The differences in the autofluorescence characteristics of the seven oral bacteria were determined by principal components analysis (PCA). The fluorescence images of the oral bacteria varied with the genus and the filter system. The three-dimensional excitation-emission matrix fluorescence spectra exhibited distinctive fluorescence features associated with intracellular fluorophores. The seven bacteria could be clearly differentiated on the PCA score plot. The findings of this study indicate that oral bacteria can be identified based on their autofluorescence characteristics. Fluorescence spectroscopy coupled with PCA can be used to detect and classify oral bacteria.
Full Text
https://onlinelibrary.wiley.com/doi/full/10.1002/jbio.201900190
DOI
10.1002/jbio.201900190
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Si-Mook(강시묵) ORCID logo https://orcid.org/0000-0003-2341-3636
Kim, Baek Il(김백일) ORCID logo https://orcid.org/0000-0001-8234-2327
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174921
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links