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The Protective Effect of a Topical Mucin Secretagogue on Ocular Surface Damage Induced by Airborne Carbon Black Exposure

Authors
 Xiangzhe Li  ;  Boram Kang  ;  Youngsub Eom  ;  Hyung Keun Lee  ;  Hyo Myung Kim  ;  Jong Suk Song 
Citation
 INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol.60(1) : 255-264, 2019 
Journal Title
 INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 
ISSN
 0146-0404 
Issue Date
2019
MeSH
Animals ; Blotting, Western ; Corneal Diseases/etiology ; Corneal Diseases/metabolism ; Corneal Diseases/prevention & control* ; Cytokines/metabolism ; Enzyme-Linked Immunosorbent Assay ; Foreign-Body Reaction/etiology ; Foreign-Body Reaction/metabolism ; Foreign-Body Reaction/prevention & control* ; Immunoglobulin E/blood ; Immunoglobulin G/blood ; L-Lactate Dehydrogenase/metabolism ; Lymph Nodes/metabolism ; Lymph Nodes/pathology ; Male ; Mucin 5AC/metabolism* ; Neck ; Particulate Matter/adverse effects* ; Polyphosphates/therapeutic use* ; Purinergic P2 Receptor Agonists/therapeutic use* ; Rats ; Rats, Sprague-Dawley ; Secretagogues/metabolism* ; Soot/adverse effects ; Tears/enzymology ; Uracil Nucleotides/therapeutic use*
Abstract
Purpose: Exposure to airborne particulate matter can induce ocular surface damage and inflammation. We evaluated the effects of a topical mucin secretagogue on the mitigation of ocular surface damage induced by exposure to airborne carbon black (CB). Methods: Sprague-Dawley rats were exposed to ambient CB for 2 hours twice daily for 5 days. Corneal staining score and tear lactic dehydrogenase (LDH) activity were measured to evaluate ocular surface damage. Serum immunoglobulin (Ig) G and IgE levels and the sizes of cervical lymph nodes were also measured. The expressions of interleukin (IL)-4, IL-17, and interferon (IFN)-γ were measured by Western blot analysis. Diquafosol tetrasodium was instilled six times a day for 5 days, and the extent of ocular surface damage was evaluated. Results: After exposure to airborne CB, the median corneal staining score and LDH activity were significantly increased. Serum IgG and IgE levels and the sizes of cervical lymph nodes were also significantly increased. Additionally, the expression of IL-4 and IFN-γ was elevated in the anterior segment of the eyeball. Furthermore, the expression of IL-4, IL-17, and IFN-γ was elevated in the cervical lymph nodes. When exposed to airborne black carbon, topical diquafosol tetrasodium significantly increased tear MUC5AC concentration and decreased tear LDH activity. Conclusions: Exposure to airborne CB induced ocular surface damage and increased proinflammatory cytokines in the eyes and cervical lymph nodes. Topical mucin secretagogues seem to have a protective effect on the ocular surface against exposure to airborne particulate matters.
Full Text
http://iovs.arvojournals.org/article.aspx?articleid=2722585
DOI
10.1167/iovs.18-25964
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Hyung Keun(이형근) ORCID logo https://orcid.org/0000-0002-1123-2136
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174721
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