Cited 3 times in
Optimal conditions for the storage of German cockroach extract
DC Field | Value | Language |
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dc.contributor.author | 박경희 | - |
dc.contributor.author | 박중원 | - |
dc.contributor.author | 육지은 | - |
dc.contributor.author | 이재현 | - |
dc.contributor.author | 이종선 | - |
dc.contributor.author | 정경용 | - |
dc.date.accessioned | 2020-02-11T06:32:10Z | - |
dc.date.available | 2020-02-11T06:32:10Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 1791-2997 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/174718 | - |
dc.description.abstract | Allergen extracts are commonly utilized for diagnosis and immunotherapy; however, the stability of protease‑rich extracts is important for a precise diagnosis and treatment efficacy. The present study determines the optimal conditions for the storage of German cockroach allergen extract. Cockroach extracts were reconstituted in four buffers: normal saline (NS), 50% glycerol in NS, 0.3% phenol in NS, or 0.3% phenol and 50% glycerol in NS. The extracts in different buffers were stored either at room temperature (18‑26˚C, RT) or refrigerated (2‑8˚C). Subsequently, the protein concentration and allergen content (Bla g 1 and Bla g 2) in the extracts were examined for the course of one year. Extract potency was estimated by inhibition ELISA. At least 90.5% protein, 94.4% Bla g 1, 65.2% Bla g 2, and 91.4% potency remained after one year when 50% glycerol NS was added to the extract with refrigeration. However, less than 13.7% protein, 17.1% Bla g 1, 0% Bla g 2 and 32.5% potency were maintained after one year when 50% glycerol NS was not added to the extract and was maintained at RT. The addition of 0.3% phenol NS did not show significant effects on extract stability. The addition of 50% glycerol NS and refrigerated storage temperature were found to be important factors for increasing the shelf life of protease‑rich cockroach extract. | - |
dc.description.statementOfResponsibility | restriction | - |
dc.language | English | - |
dc.publisher | D. A. Spandidos | - |
dc.relation.isPartOf | MOLECULAR MEDICINE REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | Optimal conditions for the storage of German cockroach extract | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | Kyoung Yong Jeong | - |
dc.contributor.googleauthor | Jongsun Lee | - |
dc.contributor.googleauthor | Ji‑Eun Yuk | - |
dc.contributor.googleauthor | Kyung Hee Park | - |
dc.contributor.googleauthor | Jae‑Hyun Lee | - |
dc.contributor.googleauthor | Jung Dong Kim | - |
dc.contributor.googleauthor | Jung‑Won Park | - |
dc.identifier.doi | 10.3892/mmr.2019.10854 | - |
dc.contributor.localId | A01427 | - |
dc.contributor.localId | A01681 | - |
dc.contributor.localId | A05833 | - |
dc.contributor.localId | A03086 | - |
dc.contributor.localId | A05836 | - |
dc.contributor.localId | A03572 | - |
dc.relation.journalcode | J02261 | - |
dc.identifier.eissn | 1791-3004 | - |
dc.identifier.pmid | 31789410 | - |
dc.identifier.url | https://www.spandidos-publications.com/mmr/21/2/953 | - |
dc.contributor.alternativeName | Park, Kyung Hee | - |
dc.contributor.affiliatedAuthor | 박경희 | - |
dc.contributor.affiliatedAuthor | 박중원 | - |
dc.contributor.affiliatedAuthor | 육지은 | - |
dc.contributor.affiliatedAuthor | 이재현 | - |
dc.contributor.affiliatedAuthor | 이종선 | - |
dc.contributor.affiliatedAuthor | 정경용 | - |
dc.citation.volume | 21 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 953 | - |
dc.citation.endPage | 958 | - |
dc.identifier.bibliographicCitation | MOLECULAR MEDICINE REPORTS, Vol.21(2) : 953-958, 2020 | - |
dc.identifier.rimsid | 63385 | - |
dc.type.rims | ART | - |
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