0 95

Cited 4 times in

A magnetic resonance tuning sensor for the MRI detection of biological targets

Authors
 Tae-Hyun Shin  ;  Sunghwi Kang  ;  Sohyeon Park  ;  Jin-sil Choi  ;  Pan Ki Kim  ;  Jinwoo Cheon 
Citation
 NATURE PROTOCOLS, Vol.13(11) : 2664-2684, 2018 
Journal Title
 NATURE PROTOCOLS 
ISSN
 1754-2189 
Issue Date
2018
MeSH
Animals ; Contrast Media/chemistry* ; Ferric Compounds/chemistry ; Ferrosoferric Oxide/chemistry ; Hydrogen-Ion Concentration ; Magnetic Resonance Imaging/methods* ; Magnetite Nanoparticles/chemistry* ; Magnetite Nanoparticles/ultrastructure ; Organometallic Compounds/chemistry* ; Succimer/chemistry ; Zinc/chemistry
Abstract
Sensors that detect specific molecules of interest in a living organism can be useful tools for studying biological functions and diseases. Here, we provide a protocol for the construction of nanosensors that can noninvasively detect biologically important targets with magnetic resonance imaging (MRI). The key operating principle of these sensors is magnetic resonance tuning (MRET), a distance-dependent phenomenon occurring between a superparamagnetic quencher and a paramagnetic enhancer. The change in distance between the two magnetic components modulates the longitudinal (T1) relaxivity of the enhancer. In this MRET sensor, distance variation is achieved by interactive linkers that undergo binding, cleavage, or folding/unfolding upon their interaction with target molecules. By the modular incorporation of suitable linkers, the MRET sensor can be applied to a wide range of targets. We showcase three examples of MRET sensors for enzymes, nucleic acid sequences, and pH. This protocol comprises three stages: (i) chemical synthesis and surface modification of the quencher, (ii) conjugation with interactive linkers and enhancers, and (iii) MRI sensing of biological targets. The entire procedure takes up to 3 d.
DOI
10.1038/s41596-018-0057-y
Appears in Collections:
5. Research Institutes (연구소) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kim, Pan Ki(김판기)
Shin, Tae-Hyun(신태현)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174461
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links