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The cytoplasmic tail of killer inhibitory receptor (KIR) associates with TCR zeta in a phosphorylation-dependent manner

 Hyun Il Cho  ;  Chae Gyu Park  ;  Jongsun Kim 
 Immunology Letters, Vol.68(2~3) : 339-345, 1999 
Journal Title
 Immunology Letters 
Issue Date
Humans ; Jurkat Cells ; Membrane Proteins/metabolism* ; Peptide Fragments/genetics ; Peptide Fragments/metabolism ; Phosphorylation ; Precipitin Tests ; Protein Binding ; Receptors, Antigen, T-Cell/metabolism* ; Receptors, Immunologic/genetics ; Receptors, Immunologic/metabolism* ; Receptors, KIR ; Receptors, KIR2DL3 ; Recombinant Fusion Proteins/metabolism
Killer inhibitory receptor (KIR) inhibits cytolytic function of killer cells by specific interaction with class I MHC molecules. The inhibitory effect mediated by KIR requires co-engagement of KIR with an activating receptor, such as TCR or FcR. This implies that KIR may function in the immediate vicinity of activating molecules, and previous studies have shown that p58 KIR is associated with TCR zeta- and FcR gamma-chain in NK cells. To better understand the molecular interaction between KIR and TCR zeta-chain, we generated a His-tag fusion protein of a p70 KIR cytoplasmic tail (His-CytKIR) and used this protein to coprecipitate TCR zeta-chain from Jurkat T cells. Western blots of the resolved coprecipitates showed that the cytoplasmic tail of KIR associates with TCR zeta in vitro. Interestingly, the association between the His-CytKIR and TCR zeta was dependent on the phosphorylation of the His-CytKIR. Unlike the unphosphorylated His-CytKIR, the phosphorylated form no longer associated with TCR zeta. However, the association was not affected by the tyrosine phosphorylation of TCR zeta. These results suggest that the cytoplasmic tail of KIR may couple to TCR zeta in a phosphorylation-dependent manner, so it could fine-tune the activation signals induced via the TCR.
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1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Kim, Jong Sun(김종선) ORCID logo https://orcid.org/0000-0002-3149-669X
Park, Chae Gyu(박채규) ORCID logo https://orcid.org/0000-0003-1906-1308
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