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Viability and enzymatic activity of cryopreserved porcine heart valve

DC FieldValueLanguage
dc.contributor.author박영환-
dc.contributor.author박종철-
dc.contributor.author이종은-
dc.date.accessioned2020-01-23T05:33:05Z-
dc.date.available2020-01-23T05:33:05Z-
dc.date.issued1999-
dc.identifier.issn0513-5796-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/174370-
dc.description.abstractFibroblast viability of a natural tissue valve for replacing a defective heart valve through allograft or xenograft has been suggested to affect its clinical durability. In this study, the cell viability and enzymatic activity of porcine heart valve leaflets were examined in regard to concerning to the preservation process [variable warm ischemic time (WIT), cold ischemic time (CIT), and cryopreservation]. Porcine heart enblocs were obtained and valve dissection was performed after 2, 12, 24, or 36 hours, in respective groups A, B, C, and D, as WIT. Each group was stored for 24 hours as CIT and cryopreserved. Leaflets were dissected from a valved conduit after each process, and cell viability and enzymatic activity in the leaflet were investigated using trypan blue staining and API ZYM kits. WIT extension significantly decreased fibroblast viability (p < 0.05, 92.25 +/- 2.7% at 2 hours, 84.9 +/- 6.7% at 12 hours, 57.0 +/- 10.2% at 24 hours, 55.9 +/- 7.9% at 36 hours), while CIT for 24 hours was also influenced significantly (p < 0.05), whereas cryopreservation demonstrated no effect on cellular viability. In enzyme activity observation, several enzymes related to lipid or nucleotide degradation (esterase, esterase lipase, particularly phosphatase, phosphohydrolase) were remarkably changed following the valve-fabrication process. After 24 hours CIT, these enzymatic activities in groups B, C and D significantly increased, but the activities decreased after cryopreservation. Particularly, both the viability and enzymatic activity showed remarkable changes after CIT in group B (WIT = 12 hours). These results suggest that WIT is more important than CIT in maintaining viability of the valve, and that completing all the cryopreservation process within 12 hours after acquisition is recommended.-
dc.description.statementOfResponsibilityopen-
dc.languageEnglish-
dc.publisherYonsei University-
dc.relation.isPartOfYonsei Medical Journal-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.subject.MESHAnimals-
dc.subject.MESHCryopreservation*-
dc.subject.MESHHeart Valves/enzymology*-
dc.subject.MESHHeart Valves/physiology*-
dc.subject.MESHSwine-
dc.subject.MESHTissue Survival/physiology*-
dc.titleViability and enzymatic activity of cryopreserved porcine heart valve-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Thoracic and Cardiovascular Surgery (흉부외과학교실)-
dc.contributor.googleauthorHwal Suh-
dc.contributor.googleauthorJong Eun Lee-
dc.contributor.googleauthorJong Chul Park-
dc.contributor.googleauthorDong Wook Han-
dc.contributor.googleauthorChee Soon Yoon-
dc.contributor.googleauthorYoung Hwan Park-
dc.contributor.googleauthorBum Koo Cho-
dc.identifier.doi10.3349/ymj.1999.40.2.184-
dc.contributor.localIdA01574-
dc.contributor.localIdA01662-
dc.contributor.localIdA01662-
dc.contributor.localIdA03146-
dc.contributor.localIdA03146-
dc.relation.journalcodeJ02813-
dc.identifier.eissn1976-2437-
dc.identifier.pmid10333724-
dc.contributor.alternativeNamePark, Young Hwan-
dc.contributor.affiliatedAuthor박영환-
dc.contributor.affiliatedAuthor박종철-
dc.contributor.affiliatedAuthor박종철-
dc.contributor.affiliatedAuthor이종은-
dc.contributor.affiliatedAuthor이종은-
dc.citation.volume40-
dc.citation.number2-
dc.citation.startPage184-
dc.citation.endPage190-
dc.identifier.bibliographicCitationYonsei Medical Journal, Vol.40(2) : 184-190, 1999-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers

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