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자궁경부암 세포의 증식에 관여하는 annexin-Ⅰ의 역할

Other Titles
 Role of annexin-I in cervical cancer cell proliferation 
Authors
 유영옥  ;  박태철  ;  남궁성은  ;  박동춘  ;  김재훈  ;  김대훈  ;  양용재  ;  이지현  ;  조성대  ;  조형권 
Citation
 Korean Journal of Obstetrics and Gynecology (대한산부인과학회잡지), Vol.42(10) : 2199-2204, 1999 
Journal Title
 Korean Journal of Obstetrics and Gynecology (대한산부인과학회잡지) 
ISSN
 0494-4755 
Issue Date
1999
Keywords
종양 ; 암 ; 자궁경부암 ; ANNEXIN-Ⅰ ; Annexin-I ; Cervical cancer ; Proliferation
Abstract
Objectives: To investigate the role of annexin-I in human cervical cancer, we evaluated the expression of annexin-I and the relation with the proliferation of cancer cells. Methods: By immunohistochemical analysis and the western blotting of annexin-I , we investigated the extent and distribution of the expression of annexin-I in cervical cancer tissues. After treating the human cancer cell lines ( SiHa and HeLa cell lines ) with tamoxifen, estradiol, and retinoic acid for 5 days to make the cells proliferate and antiproliferate, we measured the proliferation simultaneously with 3-(4,5- dimethyl thiazol- 2-yl)-2,5-diphenyl-tetr -azolium bromide (MTT) colorimetric assay and the expression level of annexin-I with flowcytometry. Results: In the immunohistochemical stains, a granular staining pattern involving the entire cytoplasm was more heavily observed in malignant lesions than in normals. In the western blotting, the antibodies against 35-kDa annexin-I appeared to react more strongly with the lysates of cancer tissues than normal and benign tissues. In SiHa and HeLa cell lines with tamoxifen and β- estradiol treatment, increased expressions of annexin-I were noted with correlated increased proliferation of cells, and with the treatments of all trans retinoic acid, decreased expressions of annexin-I were noted with correlated decreased proliferation of cells. Conclusions: The results suggest that the expression of annexin-I might correlate with cervical cancer than normal and the proliferation of cancer cells.
Full Text
http://kiss.kstudy.com/thesis/thesis-view.asp?key=1857522
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jae Hoon(김재훈) ORCID logo https://orcid.org/0000-0001-6599-7065
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174313
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