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적출한 인체 안구에서 얻은 망막색소상피세포의 환경별 및 시간별 체외 생존률

Other Titles
 In Vitro Viability of Retinal Pigment Epithelial Cells from Human Donor Eyeballs According to the Environmental Conditions and Periods 
Authors
 서경률  ;  김순현  ;  권오웅 
Citation
 Journal of the Korean Ophthalmological Society (대한안과학회지), Vol.40(2) : 481-488, 1999 
Journal Title
 Journal of the Korean Ophthalmological Society (대한안과학회지) 
ISSN
 0378-6471 
Issue Date
1999
Keywords
Age-belated macular degeneration ; Retinal pigment epithelium ; Retinal transplantation ; Environments of cell storage ; In vitro viability ; Human ; Age-related macular degeneration ; Rentinal pigment epithelium
Abstract
Retinal pigment epithelial cells(RPE) from a monolayer on the outer portion of sensory retina and have important physiologic functions that include solute transport, phagocytiosis and digestion of membrances shed from photoreceptor outer segments, and drug detoxication. Recently, transplantation of normal RPE has been proposed as a potential therapeutic modality in the surgical management of subretinal neovascularization in age related macular degeneration(ARMD). The viability of RPE at the time of transplantation is important for a good result after transplantation. This viability can be influenced by the period and environmental conditions of storage after harvest. In this study, the influence of these two factors on the viability was evaluated under controlling other remaining factors in vitro. And limitation of masimal time and optimal environmental conditions of storage were investigated. From November 1997 to February 1998, RPE cell sheets from six donor eyeballs for corneal transplantation in the department of ophthalmology of Severance Hospital were harvested and stored in -70℃, 4℃, or room temperature condotion. The viability of RPE cells at 0, 24 and 48 hours after havest was assessed and compared statistically. The viability was highest at 4℃ condition. During storage, the viability was about 95% at 24 hours for and decreased abruptly to below 90% at 48 hours. Therefore, RPE cells for transplantation are to be stored at 4℃ condition and transplantation should be performed within 24 hours after harvest.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Oh Woong(권오웅)
Seo, Kyoung Yul(서경률) ORCID logo https://orcid.org/0000-0002-9855-1980
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174230
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