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Overexpression of bcl-2, bcl-XL or hsp70 in murine cortical astrocytes reduces injury of co-cultured neurons

Authors
 Lijun Xu  ;  Jong Eun Lee  ;  , Rona G Giffard 
Citation
 Neuroscience Letters, Vol.277(3) : 193-197, 1999 
Journal Title
 Neuroscience Letters 
ISSN
 0304-3940 
Issue Date
1999
MeSH
Animals ; Astrocytes/metabolism* ; Cell Hypoxia/physiology ; Cerebral Cortex/cytology ; Cerebral Cortex/metabolism* ; Coculture Techniques ; Glucose/deficiency ; Glutamic Acid/poisoning ; HSP70 Heat-Shock Proteins/metabolism* ; Mice ; Neurons/drug effects ; Neurons/metabolism ; Neurons/pathology* ; Proto-Oncogene Proteins c-bcl-2/metabolism* ; Reference Values ; bcl-X Protein ; beta-Galactosidase/metabolism
Abstract
Astrocytes perform many functions that protect neurons during stress, including transmitter uptake, metabolic support, and protection from oxidative stress. We asked whether astrocytes overexpressing either the anti-apoptotic genes bcl-2, or bcl-XL, or the inducible heat shock protein hsp70, could better protect neurons grown with them in co-culture than normal astrocytes or astrocytes expressing beta-galactosidase. Retroviral vectors were used to express these genes in primary astrocyte cultures. After antibiotic selection to eliminate untransformed astrocytes, neurons were plated on top of the astrocytes. Overexpression of any of the three genes in astrocytes reduced neuronal injury induced by combined oxygen-glucose deprivation, or glucose deprivation. Hsp70 overexpression reduced glutamate toxicity. As none of the genes studied is thought to be secreted, the likeliest explanation for the protection observed is improved astrocyte function.
Full Text
https://www.sciencedirect.com/science/article/pii/S0304394099008824
DOI
10.1016/s0304-3940(99)00882-4
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Lee, Jong Eun(이종은) ORCID logo https://orcid.org/0000-0001-6203-7413
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/174206
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