콜린결핍 에치오닌 강화 식이로 유발한 췌장염에서 저분자 항산화물질의 효과

Abstract

Background/Aims: It has been suggested that oxygen free radicals are involved in the initiation process of acute pancreatitis. In this study, we evaluated the role of oxygen radicals and the effect of small molecular antioxidants in the development of choline-deficient ethionine supplemented (CDE) diet-induced acute pancreatitis. Methods: Acute necrotizing pancreatitis was induced in young female ICR mice (12.5 ±1.9 g) by feeding CDE diet for 48 hours. Then, the effects of antioxidant (rebamipide, N-acetyl-cysteine, allopurinol,β-carotene, and combination of α-tocopherol and ascor bate) were examined. Results: CDE diet resulted in a significant increase in serum amylase level and the concentration of pancreatic malondialdehyde (MDA). It also caused pancreatic edema and in creased proinflammatory cytokines such as TNF-α, IL-6 and IL-1 βin serum. Treatment of rebami pide, or combination of α-tocopherol and ascorbate significantly decreased the CDE diet-induced pathophysiologic deterioration of pancreas. On the other hand, allopurinol,β-carotene and N-acetyl-cysteine showed little effect. Conclusions: These results indicate that oxygen free radicals play an important role in the development of acute pancreatitis. Antioxidants may ameliorate the CDE diet induced acute pancreatitis. Further evaluation of antioxidants such as rebamipide, combination of α-tocopherol and ascorbate is necessary for possible therapeutic application.