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Identification of Chironomus kiiensis allergens, a dominant species of non-biting midges in Korea

Authors
 Tai-Soon Yong  ;  Jong-Seok Lee  ;  In-Yong Lee  ;  Soon-Jung Park  ;  Gab-Man Park  ;  Han-Il Ree  ;  Jung-Won Park  ;  Chein-Soo Hong  ;  Hae-Sim Park 
Citation
 Korean Journal of Parasitology, Vol.37(3) : 171-179, 1999 
Journal Title
 Korean Journal of Parasitology 
ISSN
 0023-4001 
Issue Date
1999
MeSH
Allergens/immunology* ; Animals ; Chironomidae/immunology* ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Immunoglobulin E/blood* ; Korea ; Mice ; Mice, Inbred BALB C ; Passive Cutaneous Anaphylaxis/immunology ; Skin Tests
Abstract
Non-biting midges are known to contain potent inhalant allergens. IgE antibody responses to the crude extract of Chironomus kiiensis adults, a dominant chironomid species in Korea, were examined. With the IgE-ELISA or passive cutaneous anaphylaxis reactions, increased levels of chironomid-specific IgE were detected in the skin test positive human sera, or immunized BALB/c mouse sera with the crude extract adsorbed to alum. IgE-immunoblot analysis showed major IgE-reacting protein band patterns, which reacted with more than 50% of the skin test positive human sera, at 110, 80, 73, 46, 40, 37, 34, and 31 kDa. The reactive band patterns were largely similar between skin test positive humans and immune BALB/c mice. However, the bands of 55, 31, 27, 26, 24, and 23 kDa were found only in sensitized humans, but not in immunized mice.
Files in This Item:
T199902383.pdf Download
DOI
10.3347/kjp.1999.37.3.171
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/173877
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