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HLA DR 양성 및 음성 Lamina Propria-like T세포 아군의 표현형 특징과 CD2 및 CD3 매개 신호전달

Other Titles
 Phenotypic Characteristics and CD2- and CD3-mediated Signal Transduction in HLA DR Positive and Negative Subse ts of Lamina Propria-like T-ce lls 
Authors
 김원호  ;  김현수  ;  이충렬  ;  문영명  ;  강진경  ;  박인서  ;  Stephan R. Targa n 
Citation
 Korean Journal of Gastroenterology (대한소화기학회지), Vol.34(5) : 609-618, 1999 
Journal Title
 Korean Journal of Gastroenterology (대한소화기학회지) 
ISSN
 1598-9992 
Issue Date
1999
Keywords
Lamina propria T-cells ; LP-like T-cells ; Phenotype ; CD2 ; Tyrosine phosphorylation
Abstract
Background/Aims: Peripheral blood T-cells co-cultured with Daudi (a irradiated B-cell line) and interleukin (IL)-2 attain a CD2 dominant cytokine secretion pattern and show tyrosine phosphorylation profile identical to freshly isolated lamina propria (LP) T-cells. Since the phenotypic characteristics and signal transduction pathway of these LP-like T-cells are largely unknown yet, we have explored the expression pattern of T-cell markers and CD2 ligation-induced tyrosine phosphorylation profile of LP-like T-cells and their HLA DR+ and DR- subsets. Methods: The LP-like T-cells were sorted into DR+ and DR- subsets by magnetic sorting. Surface markers were measured by flow cytometry. Tyrosine phosphorylation profile was investigated by Western blot. Results: The LP-like T-cells consisted of two distinct populations: approximately 55% of the cells express both HLA DR and IL-2R (DR+ LP-like T-cells) and the remainder express neither HLA DR nor IL-2R (DR- LP-like T-cells). Approximately 80% of DR+ LP-like T-cells were memory cells expressing CD45RO and 2/3 of them were activated T-cells expressing CD69, CD71 and CD30. In contrast, 50% of DR- LP-like T-cells expressed CD45RO and only 10-20% of them expressed activation markers. Tyrosine phosphorylation of p72 protein after CD2 ligation was identified only in DR- LP-like T-cells. Conclusions: The LP-like T-cells consist of two distinct subpopulation in terms of phenotype and tyrosine phosphorylation pattern according to CD2 ligation.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Won Ho(김원호) ORCID logo https://orcid.org/0000-0002-5682-9972
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/173876
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