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HLA DR 양성 및 음성 Lamina Propria-like T세포 아군의 표현형 특징과 CD2 및 CD3 매개 신호전달

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dc.contributor.author김원호-
dc.date.accessioned2020-01-10T02:06:20Z-
dc.date.available2020-01-10T02:06:20Z-
dc.date.issued1999-
dc.identifier.issn1598-9992-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/173876-
dc.description.abstractBackground/Aims: Peripheral blood T-cells co-cultured with Daudi (a irradiated B-cell line) and interleukin (IL)-2 attain a CD2 dominant cytokine secretion pattern and show tyrosine phosphorylation profile identical to freshly isolated lamina propria (LP) T-cells. Since the phenotypic characteristics and signal transduction pathway of these LP-like T-cells are largely unknown yet, we have explored the expression pattern of T-cell markers and CD2 ligation-induced tyrosine phosphorylation profile of LP-like T-cells and their HLA DR+ and DR- subsets. Methods: The LP-like T-cells were sorted into DR+ and DR- subsets by magnetic sorting. Surface markers were measured by flow cytometry. Tyrosine phosphorylation profile was investigated by Western blot. Results: The LP-like T-cells consisted of two distinct populations: approximately 55% of the cells express both HLA DR and IL-2R (DR+ LP-like T-cells) and the remainder express neither HLA DR nor IL-2R (DR- LP-like T-cells). Approximately 80% of DR+ LP-like T-cells were memory cells expressing CD45RO and 2/3 of them were activated T-cells expressing CD69, CD71 and CD30. In contrast, 50% of DR- LP-like T-cells expressed CD45RO and only 10-20% of them expressed activation markers. Tyrosine phosphorylation of p72 protein after CD2 ligation was identified only in DR- LP-like T-cells. Conclusions: The LP-like T-cells consist of two distinct subpopulation in terms of phenotype and tyrosine phosphorylation pattern according to CD2 ligation.-
dc.description.statementOfResponsibilityopen-
dc.languageKorean Journal of Gastroenterology-
dc.publisherKorean Journal of Gastroenterology-
dc.relation.isPartOfKorean Journal of Gastroenterology-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.titleHLA DR 양성 및 음성 Lamina Propria-like T세포 아군의 표현형 특징과 CD2 및 CD3 매개 신호전달-
dc.title.alternativePhenotypic Characteristics and CD2- and CD3-mediated Signal Transduction in HLA DR Positive and Negative Subse ts of Lamina Propria-like T-ce lls-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학교실)-
dc.contributor.googleauthor김원호-
dc.contributor.googleauthor김현수-
dc.contributor.googleauthor이충렬-
dc.contributor.googleauthor문영명-
dc.contributor.googleauthor강진경-
dc.contributor.googleauthor박인서-
dc.contributor.googleauthorStephan R. Targa n-
dc.contributor.localIdA00774-
dc.relation.journalcodeJ02015-
dc.identifier.eissn2233-6869-
dc.subject.keywordLamina propria T-cells-
dc.subject.keywordLP-like T-cells-
dc.subject.keywordPhenotype-
dc.subject.keywordCD2-
dc.subject.keywordTyrosine phosphorylation-
dc.contributor.alternativeNameKim, Won Ho-
dc.contributor.affiliatedAuthor김원호-
dc.citation.volume34-
dc.citation.number5-
dc.citation.startPage609-
dc.citation.endPage618-
dc.identifier.bibliographicCitationKorean Journal of Gastroenterology, Vol.34(5) : 609-618, 1999-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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