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HLA DR 양성 및 음성 Lamina Propria-like T세포 아군의 표현형 특징과 CD2 및 CD3 매개 신호전달
DC Field | Value | Language |
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dc.contributor.author | 김원호 | - |
dc.date.accessioned | 2020-01-10T02:06:20Z | - |
dc.date.available | 2020-01-10T02:06:20Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 1598-9992 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/173876 | - |
dc.description.abstract | Background/Aims: Peripheral blood T-cells co-cultured with Daudi (a irradiated B-cell line) and interleukin (IL)-2 attain a CD2 dominant cytokine secretion pattern and show tyrosine phosphorylation profile identical to freshly isolated lamina propria (LP) T-cells. Since the phenotypic characteristics and signal transduction pathway of these LP-like T-cells are largely unknown yet, we have explored the expression pattern of T-cell markers and CD2 ligation-induced tyrosine phosphorylation profile of LP-like T-cells and their HLA DR+ and DR- subsets. Methods: The LP-like T-cells were sorted into DR+ and DR- subsets by magnetic sorting. Surface markers were measured by flow cytometry. Tyrosine phosphorylation profile was investigated by Western blot. Results: The LP-like T-cells consisted of two distinct populations: approximately 55% of the cells express both HLA DR and IL-2R (DR+ LP-like T-cells) and the remainder express neither HLA DR nor IL-2R (DR- LP-like T-cells). Approximately 80% of DR+ LP-like T-cells were memory cells expressing CD45RO and 2/3 of them were activated T-cells expressing CD69, CD71 and CD30. In contrast, 50% of DR- LP-like T-cells expressed CD45RO and only 10-20% of them expressed activation markers. Tyrosine phosphorylation of p72 protein after CD2 ligation was identified only in DR- LP-like T-cells. Conclusions: The LP-like T-cells consist of two distinct subpopulation in terms of phenotype and tyrosine phosphorylation pattern according to CD2 ligation. | - |
dc.description.statementOfResponsibility | open | - |
dc.language | Korean Journal of Gastroenterology | - |
dc.publisher | Korean Journal of Gastroenterology | - |
dc.relation.isPartOf | Korean Journal of Gastroenterology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.title | HLA DR 양성 및 음성 Lamina Propria-like T세포 아군의 표현형 특징과 CD2 및 CD3 매개 신호전달 | - |
dc.title.alternative | Phenotypic Characteristics and CD2- and CD3-mediated Signal Transduction in HLA DR Positive and Negative Subse ts of Lamina Propria-like T-ce lls | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학교실) | - |
dc.contributor.googleauthor | 김원호 | - |
dc.contributor.googleauthor | 김현수 | - |
dc.contributor.googleauthor | 이충렬 | - |
dc.contributor.googleauthor | 문영명 | - |
dc.contributor.googleauthor | 강진경 | - |
dc.contributor.googleauthor | 박인서 | - |
dc.contributor.googleauthor | Stephan R. Targa n | - |
dc.contributor.localId | A00774 | - |
dc.relation.journalcode | J02015 | - |
dc.identifier.eissn | 2233-6869 | - |
dc.subject.keyword | Lamina propria T-cells | - |
dc.subject.keyword | LP-like T-cells | - |
dc.subject.keyword | Phenotype | - |
dc.subject.keyword | CD2 | - |
dc.subject.keyword | Tyrosine phosphorylation | - |
dc.contributor.alternativeName | Kim, Won Ho | - |
dc.contributor.affiliatedAuthor | 김원호 | - |
dc.citation.volume | 34 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 609 | - |
dc.citation.endPage | 618 | - |
dc.identifier.bibliographicCitation | Korean Journal of Gastroenterology, Vol.34(5) : 609-618, 1999 | - |
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