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Streptococcus mutans에 대한 생강과 천연추출물의 광역학 항균효과

Other Titles
 Antibacterial effect of Zingiberaceae extracts mediated photodynamic therapy on Streptococcus mutans 
 황혜림  ;  강시묵  ;  이은송  ;  김백일 
 Journal of the Korean Dental Association (대한치과의사협회지), Vol.57(10) : 560-568, 2019 
Journal Title
Journal of the Korean Dental Association(대한치과의사협회지)
Issue Date
photodynamic therapy ; natural photosensitizer ; Quantitative Light-induced fluorescence
Purpose: This study evaluated the antibacterial effects of curcuma, ginger, and finger root extracts in water-soluble powder on planktonic Streptococcus mutans(S.mutans), which is known to cause dental caries, in order to confirm whether these extracts could perform as photosensitizers for the effects of photodynamic therapy (PDT). Methods: This study used the strain of streptococcus mutans ATCC 25175 distributed by the Korean Collection for Type Cultures of the Korea Research Institute of Bioscience & Biotechnology. Commercial edible curcuma, ginger and finger root were used as the natural extracts for the use of photosensitizer. To extract organic solvent, 3 g of each powder was mixed in $30m{\ell}$ 수식 이미지 of dimethyl sulfoxide (DMSO, VWR, Germany) before extraction. $1.8m{\ell}$ 수식 이미지 of the photosensitizer solution, manufactured in the concentrations of 5, 0.5, and $0.05mg/m{\ell}$ 수식 이미지, was mixed with $0.2m{\ell}$ 수식 이미지 of the S. mutans culture medium that had been cultured for 2 days. To induce the photodynamic reaction, Qraycam (AIOBIO, Seoul, Korea) equipped with 405 nm LED was used to expose light for 5 minutes to irradiate 59 nW energy for 300 seconds. Results: Compared with the case with no light, a higher photodynamic therapeutic effect was confirmed with $0.05mg/m{\ell}$ 수식 이미지 curcuma powder extract, the concentration of $0.5mg/m{\ell}$ 수식 이미지 and LED light of 405 nm wavelength (p=0.000, p=0.003). $0.05mg/m{\ell}$ 수식 이미지 of curcuma powder extract and the concentration of $0.5mg/m{\ell}$ 수식 이미지 showed 100% antibacterial effect when exposed to light, whereas the concentration of $5mg/m{\ell}$ 수식 이미지 showed 11.95% antibacterial effect. When exposed to light, $0.05mg/m{\ell}$ 수식 이미지 of ginger powder extract showed an antibacterial effect which didn't statistically decrease. The concentrations of $0.5mg/m{\ell}$ 수식 이미지 and $5mg/m{\ell}$ 수식 이미지 did not show any antibacterial effects. As a result of examining any photodynamic therapeutic effects of finger root powder extract on S. mutans, no statistically significant effect was found. Conclusion: The curcuma powder extract is expected to perform as a photosensitizer. Even though belonging to the same ginger family, ginger powder and finger root powder seem difficult to perform as photosensitizer.
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Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Preventive Dentistry and Public Oral Health (예방치과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kang, Si-Mook(강시묵) ORCID logo https://orcid.org/0000-0003-2341-3636
Kim, Baek Il(김백일) ORCID logo https://orcid.org/0000-0001-8234-2327
Lee, Eun Song(이은송) ORCID logo https://orcid.org/0000-0002-2949-4783
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