Streptococcus mutans에 대한 생강과 천연추출물의 광역학 항균효과

Abstract

Purpose: This study evaluated the antibacterial effects of curcuma, ginger, and finger root extracts in water-soluble powder on planktonic Streptococcus mutans(S.mutans), which is known to cause dental caries, in order to confirm whether these extracts could perform as photosensitizers for the effects of photodynamic therapy (PDT). Methods: This study used the strain of streptococcus mutans ATCC 25175 distributed by the Korean Collection for Type Cultures of the Korea Research Institute of Bioscience & Biotechnology. Commercial edible curcuma, ginger and finger root were used as the natural extracts for the use of photosensitizer. To extract organic solvent, 3 g of each powder was mixed in $30m{\ell}$ 수식 이미지 of dimethyl sulfoxide (DMSO, VWR, Germany) before extraction. $1.8m{\ell}$ 수식 이미지 of the photosensitizer solution, manufactured in the concentrations of 5, 0.5, and $0.05mg/m{\ell}$ 수식 이미지, was mixed with $0.2m{\ell}$ 수식 이미지 of the S. mutans culture medium that had been cultured for 2 days. To induce the photodynamic reaction, Qraycam (AIOBIO, Seoul, Korea) equipped with 405 nm LED was used to expose light for 5 minutes to irradiate 59 nW energy for 300 seconds. Results: Compared with the case with no light, a higher photodynamic therapeutic effect was confirmed with $0.05mg/m{\ell}$ 수식 이미지 curcuma powder extract, the concentration of $0.5mg/m{\ell}$ 수식 이미지 and LED light of 405 nm wavelength (p=0.000, p=0.003). $0.05mg/m{\ell}$ 수식 이미지 of curcuma powder extract and the concentration of $0.5mg/m{\ell}$ 수식 이미지 showed 100% antibacterial effect when exposed to light, whereas the concentration of $5mg/m{\ell}$ 수식 이미지 showed 11.95% antibacterial effect. When exposed to light, $0.05mg/m{\ell}$ 수식 이미지 of ginger powder extract showed an antibacterial effect which didn't statistically decrease. The concentrations of $0.5mg/m{\ell}$ 수식 이미지 and $5mg/m{\ell}$ 수식 이미지 did not show any antibacterial effects. As a result of examining any photodynamic therapeutic effects of finger root powder extract on S. mutans, no statistically significant effect was found. Conclusion: The curcuma powder extract is expected to perform as a photosensitizer. Even though belonging to the same ginger family, ginger powder and finger root powder seem difficult to perform as photosensitizer.